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Coupling of Spinosad Fermentation and Separation Process via Two-Step Macroporous Resin Adsorption Method

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Abstract

In this paper, a two-step resin adsorption technology was investigated for spinosad production and separation as follows: the first step resin addition into the fermentor at early cultivation period to decrease the timely product concentration in the broth; the second step of resin addition was used after fermentation to adsorb and extract the spinosad. Based on this, a two-step macroporous resin adsorption-membrane separation process for spinosad fermentation, separation, and purification was established. Spinosad concentration in 5-L fermentor increased by 14.45 % after adding 50 g/L macroporous at the beginning of fermentation. The established two-step macroporous resin adsorption-membrane separation process got the 95.43 % purity and 87 % yield for spinosad, which were both higher than that of the conventional crystallization of spinosad from aqueous phase that were 93.23 and 79.15 % separately. The two-step macroporous resin adsorption method has not only carried out the coupling of spinosad fermentation and separation but also increased spinosad productivity. In addition, the two-step macroporous resin adsorption-membrane separation process performs better in spinosad yield and purity.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (Nos. 21076148 and 31270087) and the Plan for Tianjin Science and Technology Support (No 11ZCKFSY0100).

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The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

      Fanglong Zhao, Chuanbo Zhang, Jing Yin, Yueqi Shen & Wenyu Lu

    2. Key Laboratory of system bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People’s Republic of China

      Fanglong Zhao, Chuanbo Zhang, Jing Yin, Yueqi Shen & Wenyu Lu

    3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People’s Republic of China

      Fanglong Zhao, Chuanbo Zhang, Jing Yin, Yueqi Shen & Wenyu Lu

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    1. Fanglong Zhao
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    Correspondence to Wenyu Lu.

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    Fanglong Zhao and Chuanbo Zhang contributed equally to this work.

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    Zhao, F., Zhang, C., Yin, J. et al. Coupling of Spinosad Fermentation and Separation Process via Two-Step Macroporous Resin Adsorption Method. Appl Biochem Biotechnol 176, 2144–2156 (2015). https://doi.org/10.1007/s12010-015-1704-1

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