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Improvement of Saccharopolyspora spinosa and the Kinetic Analysis for Spinosad Production

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Abstract

In this paper, a new spinosad-producing mutant UV-42-13 was obtained by employing rhamnose and sodium propionate resistant selection strategies in series with UV irradiation. Spinosad production of the mutant was 125.3 mg/L, improved 285.5% compared with that of the wild strain (32.5 mg/L).The results of experiment on tolerance of propyl alcohol addition showed that the tolerant ability to precursor was higher. The precursor-resistant ability of the mutant improved through tolerance experiment by adding propyl alcohol, and the spinosad production was greatly increased. The kinetic models for biomass, substrate consumption, and spinosad production of mutant strain and wild strain were studied by conducting batch fermentation in the shaking flask. The result showed that the kinetic models could describe the fermentation process of spinosad producing well.

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Authors and Affiliations

  1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Yan Liang, Wenyu Lu & Jianping Wen

Authors
  1. Yan Liang
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  2. Wenyu Lu
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  3. Jianping Wen
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Correspondence to Jianping Wen.

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Liang, Y., Lu, W. & Wen, J. Improvement of Saccharopolyspora spinosa and the Kinetic Analysis for Spinosad Production. Appl Biochem Biotechnol 152, 440–448 (2009). https://doi.org/10.1007/s12010-008-8281-5

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  • DOI: https://doi.org/10.1007/s12010-008-8281-5

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