Skip to main content
SpringerLink
Log in

Protopine Production by Fumaria Cell Suspension Cultures: Effect of Light

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Protopine biosynthesis in Fumaria rostellata and Fumaria officinalis cell suspensions was investigated. For the first time, we reported for calli and cell suspensions obtained from F. rostellata and F. officinalis. Callus induction was initiated on a Murashige and Skoog medium, supplemented with sucrose and various concentrations of plant growth regulators: 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The best morphological characteristics, growth behavior, and protopine biosynthesis were observed for two callus lines (5FRL14 and 12FOL1) cultivated under submerged conditions, at low concentration of 2,4-D (0.2 and 0.5 mg/L) and higher concentration of BAP (2.0 and 3.0 mg/L). The maximal yield of protopine was accumulated from cell suspension of F. rostellata (line 5FRL14) cultivated under illumination—49.6 mg/L. Time courses of utilization of sucrose, ammonium, nitrate, and phosphate ions in cultural liquid and acetylcholinesterase inhibitory activity of alkaloid extracts of studied suspensions are also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Saeed, S. A., Gilani, A. H., Majoo, R. U., & Shah, B. H. (1997). Anti-thrombotic and anti-inflammatory activities of protopine. Pharmacological Research, 36(1), 1–7.

    Article  CAS  Google Scholar 

  2. Shen, G. Y., Chen, W. R., Midtgaard, J., Shepherd, G. M., & Hines, M. L. (1999). Computational analysis of action potential initiation in mitral cell soma and dendrites based on dual patch recordings. Journal of Neurophysiology, 82(6), 3006–3020.

    CAS  Google Scholar 

  3. Li, B., Wu, Q., Shi, J. S., Sun, A. S., & Huang, X. N. (2005). Effects of protopine on intracellular calcium and the PKC activity of rat aorta smooth muscle. Acta Pharmacologica Sinica, 57, 240–246.

    CAS  Google Scholar 

  4. Cosar, G., Bilgehan, H., & Gozler, T. (1981). The antibacterial effects of some alkaloids isolated from Glaucium fravum Crantz. Mikrobiyoloji Bülteni, 15, 105–109.

    CAS  Google Scholar 

  5. Ustunes, L., Laekeman, G. M., Gozler, B., Vlietinck, A. J., Ozer, A., & Herman, A. G. (1988). In vitro study of the anticholinergic and antihistaminic activities of protopine and some derivates. Journal of Natural Products, 51, 1021–1022.

    Article  CAS  Google Scholar 

  6. Lee, K. H., Huh, J. W., Choi, M. M., Yoon, S. Y., Yang, S. J., Hong, H. N., & Cho, S. W. (2005). Regulation of glutamate level in rat brain through activation of glutamate dehydrogenase by Corydalis ternate. Experimental and Molecular Medicine, 37(4), 371–377.

    Article  CAS  Google Scholar 

  7. Hu, X. Y., Sun, A. S., Yu, L. M., & Wu, Q. (2005). Effects of Corydalis ambailis migo total alkaloids on experimental cerebral ischemia. Zhong Xi Yi Jie He Xue Bao, 3, 46–49.

    Article  CAS  Google Scholar 

  8. Suau, R., Cabezudo, B., Rico, R., Nájera, F., & López-Romero, J. M. (2002). Direct determination of alkaloid contents in Fumaria species by GC-MS. Phytochemical Analysis, 13, 363–367.

    Article  CAS  Google Scholar 

  9. Suau, R., Cabezudo, B., Rico, R., López-Romero, J. M., & Náera, F. (2002). Alkaloids from Fumaria sepium and Fumaria agrarian. Biochemical Systematics and Ecology, 30, 263–265.

    Article  CAS  Google Scholar 

  10. Assyov, B., Petrova, A., Dimitrov, D., & Vassilev, R. (2012). Conspectus of the Bulgarian Vascular flora. Distribution maps and floristic elements (4th ed.). Sofia: Bulgarian Biodiversity Foundation.

    Google Scholar 

  11. Tanahashi, T., & Zenk, M. H. (1985). Isoquinoline alkaloids from cell suspension cultures of Fumaria capreolata. Plant Cell Reports, 4(2), 96–99.

    Article  CAS  Google Scholar 

  12. Sousek, J., Guèdon, D., Adam, T., Bochoràkova, H., Tàborskà, E., Vàlka, I., & Simànek, V. (1999). Alkaloids and organic acids content of eight Fumaria Species. Phytochemical Analysis, 10(1), 6–11.

    Article  CAS  Google Scholar 

  13. Maiza-Benabdesselam, F., Chibane, M., Madani, K., Max, H., & Adach, S. (2007). Determination of isoquinoline alkaloids contents in two Algerian species of Fumaria (Fumaria capreolata and Fumaria bastardy). African Journal of Biotechnology, 6(21), 2487–2492.

    CAS  Google Scholar 

  14. Ivanov, I., Vrancheva, R., Marchev, A., Petkova, N., Aneva, I., Denev, P., Georgiev, V., & Pavlov, A. (2014). Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. International Journal of Current Microbiology and Applied Science, 3(2), 296–306.

    CAS  Google Scholar 

  15. Xu, L. F., Chu, W. J., Qing, X. Y., Li, S., Wang, X. S., Qing, G. W., Fei, J., & Guo, L. H. (2006). Protopine inhibits serotonin transporter and noradrenaline transporter and has the antidepressant-like effect in mice models. Neuropharmacology, 50(8), 934–940.

    Article  CAS  Google Scholar 

  16. Orhan, I., Özçelik, B., Karaoğlu, T., & Sener, B. (2007). Antiviral and antimicrobial profiles of selected isoquinoline alkaloids from Fumaria and Corydalis species. Zeitschrift für Naturforschung. Section C, 62(1–2), 19–26.

    CAS  Google Scholar 

  17. Rathi, A., Srivastava, A. K., Shirwaikar, A., Rawat, A. K. S., & Mehrotra, S. (2008). Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine, 15(6–7), 470–477.

    Article  CAS  Google Scholar 

  18. Vrancheva, R., Ivanov, I., Marchev, A., & Pavlov, A. (2012). Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method. Journal of Biomedicine and Biotechnology, 1(3), 255–259.

    Google Scholar 

  19. Paltinenan, R., Toiu, A., Wauters, J. N., Frederich, M., Tits, M., Angelot, L., Tamas, M., & Crisan, G. (2013). Indentification and determination of alkaloids in Fumaria Species from Romania. Digest Journal of Nanomaterials and Biostructures, 8(2), 817–824.

    Google Scholar 

  20. Ananga, A., Georgiev, V., Ochieng, J. W., Phills, B., & Tsolova, V. (2013). Production of anthocyanins in grape cell cultures: A potential source of raw material for pharmaceutical, food, and cosmetic industries. In B. Sladonja (Ed.), The Mediterranean Genetic Code - Grapevine and Olive (pp. 247–287). Intech.

  21. Murthy, H. N., Dandin, V. S., Zhong, J. J., & Paek, K. Y. (2014). Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In N. Murthy, J. Zhong, & Y. Paek (Eds.), Production of biomass and bioactive compounds using bioreactor technology (pp. 471–508). Netherlands: Springer.

    Chapter  Google Scholar 

  22. Dixon, R. A. (1985). Isolation and maintenance of callus and cell suspension cultures in plant cell culture. In R. A. Dixon (Ed.), A practical approach (pp. 1–20). Oxford.

  23. Pavlov, A., Georgiev, V., & Ilieva, M. (2005). Betalain biosynthesis by red beet (Beta vulgaris L.) hairy root culture. Process Biochemistry, 40(5), 1531–1533.

    Article  CAS  Google Scholar 

  24. Petkova, N., Vrancheva, R., Denev, P., Ivanov, I., & Pavlov, A. (2013). HPLC-RID method for determination of inulin and fructooligosaccharides. Conference: XI National Conference “Natural Sciences 2013, “Bishop Konstantin Preslavski” Shumen University, pp 99–107.

  25. Perry, N. S. L., Houghton, P. J., Theobald, A., Jenner, P., & Perry, E. K. (2000). In vitro inhibition of human erythrocyte acetylcholinesterase by Salvia lavandulaefolia essential oil and constituent terpenes. Journal of Pharmacy and Pharmacology, 52(7), 895–902.

    Article  CAS  Google Scholar 

  26. Georgiev, V., Marchev, A., Haas, C., Weber, J., Nikolova, M., Bley, T., & Pavlov, A. (2011). Production of oleanolic and ursolic acids by callus cultures of Salvia tomentosa Mill. Biotechnology and Biotechnological Equipment, 25(1), 34–38.

    Article  Google Scholar 

  27. Georgiev, V., Ivanov, I., & Pavlov, A. (2010). Obtaining and selection of Pancratium maritimum L. In vitro cultures with acetylcholinesterase inhibitory action. Biotechnology and Biotechnological Equipment, 24, 149–153.

    Article  Google Scholar 

  28. Wilson, S. A., & Roberts, S. C. (2012). Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules. Plant Biotechnology Journal, 10(3), 49–268.

    Article  Google Scholar 

  29. Weathers, P. J., Towler, M. J., & Xu, J. (2010). Bench to batch: advances in plant cell culture for producing useful products. Applied Microbiology and Biotechnology, 85(5), 1339–1351.

    Article  CAS  Google Scholar 

  30. Ali, M., & Abbasi, B. H. (2014). Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology, 140, 223–227.

    Article  CAS  Google Scholar 

  31. Zhao, J., Zhu, W. H., & Hu, O. (2001). Enhanced catharanthine production in catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors. Enzyme and Microbial Technology, 28, 673–681.

    Article  CAS  Google Scholar 

  32. Tzin, V., & Galili, G. (2010). The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. In G. Jander (Ed.), The arabidopsis book (pp. 2–18). Minneapolis: The American Society of Plant Biologists.

    Google Scholar 

  33. Pavlov, A., Berkov, S., Weber, J., & Bley, T. (2009). Hyoscyamine biosynthesis in Datura stramonium hairy root in vitro systems with different ploidy levels. Applied Biochemistry and Biotechnology, 153, 210–225.

    Article  Google Scholar 

  34. Sung, S. S., Kormanik, P. P., & Xu, P. D. (1989). Sucrose metabolic pathways in sweetgum and pecan seedlings. Tree Physiology, 5, 39–52.

    Article  CAS  Google Scholar 

  35. Baena-Gonzalez, E., Rolland, F., Thevelein, J. M., & Sheen, J. (2007). A central integrator of transcription networks in plant stress and energy signalling. Nature, 448, 938–942.

    Article  CAS  Google Scholar 

  36. Berkov, S., Bastida, J., Nikolova, M., Viladomat, F., & Codina, C. (2008). Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts. Phytochemical Analysis, 19(5), 411–419.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank for the financial support of this research by the Bulgarian Science Foundation, Bulgarian Ministry of Education and Science under contract number DMU 03/77 - 2011.

Conflict of Interest

The authors declare that there is no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Organic Chemistry, University of Food Technologies, 4002, Plovdiv, Bulgaria

    Lidiya Georgieva, Ivan Ivanov & Panteley Denev

  2. Department of Applied Microbiology, Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Science, 4000, Plovdiv, Bulgaria

    Andrey Marchev & Atanas Pavlov

  3. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Science, 1113, Sofia, Bulgaria

    Ina Aneva

  4. Center for Viticulture and Small Fruit Research, College of Agriculture and Food Science, Florida A&M University, 6505 Mahan Drive, Tallahassee, FL, 32317, USA

    Vasil Georgiev

  5. Department of Analytical Chemistry, University of Food Technologies, 4002, Plovdiv, Bulgaria

    Atanas Pavlov

Authors
  1. Lidiya Georgieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ivan Ivanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrey Marchev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ina Aneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Panteley Denev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vasil Georgiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Atanas Pavlov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atanas Pavlov.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Georgieva, L., Ivanov, I., Marchev, A. et al. Protopine Production by Fumaria Cell Suspension Cultures: Effect of Light. Appl Biochem Biotechnol 176, 287–300 (2015). https://doi.org/10.1007/s12010-015-1574-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-015-1574-6

Keywords

Search

Navigation