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High-level Production of Creatine Amidinohydrolase from Arthrobacter nicotianae 23710 in Escherichia coli

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Abstract

In the present study, the gene encoding creatinase was amplified from Arthrobacter nicotianae 23710 (CICC) and functionally overexpressed in Escherichia coli. By applying a two-stage temperature control strategy, the production of creatinase was increased up to 61.3 U/mL in 3-L fermentor with a high productivity of 6.1 U/mL/h. The recombinant creatinase shows excellent resistance to the chelating agent EDTA, the surfactants (Tween 20, Tween 80, and Triton X-100) and the common preservative NaN3 (20 mM). High-level expression of the recombinant creatinase will contribute to its application in clinical diagnosis of renal function.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (863 Program, 2011AA100905), Program for Changjiang Scholars and Innovative Research Team in University (no. IRT1135), the National Science Foundation for Post-doctoral Scientists of China (2013 M540414), the Jiangsu Planned Projects for Postdoctoral Research Funds (1301010B), and the 111 Project.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Jun Dai, Linpei Zhang & Zhen Kang

  2. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Jun Dai, Linpei Zhang, Zhen Kang, Jian Chen & Guocheng Du

  3. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

  4. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Guocheng Du

Authors
  1. Jun Dai
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  2. Linpei Zhang
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  3. Zhen Kang
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  4. Jian Chen
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  5. Guocheng Du
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Correspondence to Zhen Kang.

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Dai, J., Zhang, L., Kang, Z. et al. High-level Production of Creatine Amidinohydrolase from Arthrobacter nicotianae 23710 in Escherichia coli . Appl Biochem Biotechnol 175, 2564–2573 (2015). https://doi.org/10.1007/s12010-014-1460-7

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