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Biohydrogen Production in an AnSBBR Treating Glycerin-Based Wastewater: Effects of Organic Loading, Influent Concentration, and Cycle Time

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Abstract

This study evaluated the influence of the applied volumetric organic load on biohydrogen production in an anaerobic sequencing batch biofilm reactor (AnSBBR) with 3.5 L of liquid medium and treating 1.5 L of glycerin-based wastewater per cycle at 30 °C. Six applied volumetric organic loads (AVOLCT) were generated from the combination of cycle periods (3 and 4 h) and influent concentrations (3000, 4000, and 5000 mg chemical oxygen demand (COD) L−1), with values ranging from 7565 to 16,216 mg COD L−1 day−1. No clear relationship was found between the applied volumetric organic load and the hydrogen production. However, the highest hydrogen molar production (MPr 67.5 mol H2 m−3 day−1) was reached when the reactor was operated with a cycle period of 4 h and an influent concentration of 5000 mg COD L−1 (AVOLCT 12,911 mg COD L−1 day−1). This condition also reached the highest molar yield per applied load based on the organic matter (MYALC,m 21.1 mol H2 kg COD−1). In addition, the pretreatment of the sludge/inoculum was found to not influence the productivity/yield of the process, and the use of crude glycerol as a sole source of carbon exhibited a clear disadvantage for hydrogen production compared to pure glycerol. The AnSBBR used for the hydrogen production experiments operated with pure glycerol as a sole carbon source exhibited important practical potential.

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Abbreviations

ASOLCT :

Applied specific organic load based on organic matter (kg COD m−3 day−1)

AVOLCT :

Applied volumetric organic load based on organic matter (kg COD m−3 day−1)

C CF :

Concentration based on organic matter for filtered samples in the effluent (mg COD L−1)

C CT :

Concentration based on organic matter for unfiltered samples in the effluent (mg COD L−1)

C CT,I :

Concentration based on organic matter for unfiltered samples in the influent (mg COD L−1)

C H2 :

Molar concentration (not quantity) of hydrogen at normal conditions of temperature and pressure (mmol-NCTP L-1)

C CH4 :

Molar concentration (not quantity) of methane at normal conditions of temperature and pressure (mmol-NCTP L-1)

C X :

Concentration of biomass in the reactor in total volatile solids per volume of liquid (g TVS L−1)

C′X :

Concentration of biomass in the reactor in total volatile solids per mass of support (g TVS g support−1)

M TVS :

Total biomass in the reactor in total volatile solids (g TVS)

MPr:

Daily molar productivity of hydrogen (mol H2 m−3 day−1)

MYALC,m :

Molar yield per applied load based on organic matter expressed in kilograms (mol H2 kg COD−1)

MYRLC,m :

Molar yield per removed load based on organic matter expressed in kilograms (mol H2 kg COD−1)

N :

Number of cycles per day (cycle day−1)

n H2 :

Daily molar production of hydrogen (mol day−1)

RSOLCF :

Removed volumetric specific load based on organic matter (kg COD g TVS−1 day−1)

RVOLCF :

Removed specific organic load based on organic matter (kg COD m−3 day−1)

SB:

Sodium bicarbonate

SS:

Solutions of the salts specified in “Wastewater”

SMPr:

Daily specific molar productivity of hydrogen (mol H2 kg TVS−1 day−1)

t C :

Cycle time (h cycle−1)

TA:

Total alkalinity (mg CaCO3 L−1)

TS:

Total solids concentration (mg L−1)

TSS:

Total suspended solids concentration (mg L−1)

TVA:

Total volatile acids (mg HAc L−1)

TVS:

Total volatile solids concentration (mg L−1)

VSS:

Volatile suspended solids concentration (mg L−1)

V F :

Volume of wastewater fed during the cycle (L cycle−1)

V H2 :

Daily volumetric production of hydrogen (mol day−1)

V R :

Volume of liquid medium in the reactor (L)

V G :

Normal volume of biogas (H2, CO2, and CH4) produced along a cycle (NmL cycle−1)

ε CF :

Removal efficiency based on organic matter for filtered samples (%)

ε CT :

Removal efficiency based on organic matter for unfiltered samples (%)

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Acknowledgments

This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil, 2009/15.984-0), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), and the Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES, Brazil).

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Authors and Affiliations

  1. Escola de Engenharia de São Carlos, Universidade de São Paulo (USP), Av. Trabalhador São-Carlense 400, São Carlos, SP, 13566-590, Brazil

    I. S. Moncayo Bravo, G. Lovato & M. Zaiat

  2. Escola de Engenharia Mauá, Instituto Mauá de Tecnologia (IMT), Praça Mauá 1, São Caetano do Sul, SP, 09580-900, Brazil

    J. A. D. Rodrigues & S. M. Ratusznei

Authors
  1. I. S. Moncayo Bravo
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  2. G. Lovato
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  3. J. A. D. Rodrigues
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  4. S. M. Ratusznei
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  5. M. Zaiat
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Corresponding author

Correspondence to J. A. D. Rodrigues.

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Bravo, I.S.M., Lovato, G., Rodrigues, J.A.D. et al. Biohydrogen Production in an AnSBBR Treating Glycerin-Based Wastewater: Effects of Organic Loading, Influent Concentration, and Cycle Time. Appl Biochem Biotechnol 175, 1892–1914 (2015). https://doi.org/10.1007/s12010-014-1421-1

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  • DOI: https://doi.org/10.1007/s12010-014-1421-1

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