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Fungal Pretreatment by Phanerochaete chrysosporium for Enhancement of Biogas Production from Corn Stover Silage

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Abstract

Corn stover silage (CSS) was pretreated by Phanerochaete chrysosporium in solid-state fermentation (SSF), to enhance methane production via subsequent anaerobic digestion (AD). Effects of washing of corn stover silage (WCSS) on the lignocellulosic biodegradability in the fungal pretreatment step and on methane production in the AD step were investigated with comparison to the CSS. It was found that P. chrysosporium had the degradation of cellulose, hemicellulose, and lignin of CSS up to 19.9, 32.4, and 22.6 %, respectively. Consequently, CSS pretreated by 25 days achieved the highest methane yield of 265.1 mL/g volatile solid (VS), which was 23.0 % higher than the untreated CSS. However, the degradation of cellulose, hemicellulose, and lignin in WCSS after 30 days of SSF increased to 45.9, 48.4, and 39.0 %, respectively. Surface morphology and Fourier-transform infrared spectroscopy analyses also demonstrated that the WCSS improved degradation of cell wall components during SSF. Correspondingly, the pretreatment of WCSS improved methane production by 19.6 to 32.6 %, as compared with untreated CSS. Hence, washing and reducing organic acids (such as lactic acid, acetic acid, propionic acid, and butyric acid) present in CSS has been proven to further improve biodegradability in SSF and methane production in the AD step.

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Acknowledgments

This work was supported by grants from the project of “Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, China,” the Chinese Universities Scientific Fund (No. 2014JD034 and 2013YJ007) and Beijing Municipal Science and Technology Projects (No. D141100001214001 and D141100001214002). We likewise greatly appreciate the critical and constructive comments from the anonymous reviewers, which have helped improve this manuscript.

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Authors and Affiliations

  1. Key Laboratory of Clean Utilization Technology for Renewable Energy in Ministry of Agriculture, College of Engineering, China Agricultural University, Beijing, 100083, People’s Republic of China

    Shan Liu, Shubiao Wu & Renjie Dong

  2. Key Laboratory of Development and Application of Rural Renewable Energy in Ministry of Agriculture, Biogas Scientific Research Institute of the Ministry of Agriculture, Chengdu, 610041, People’s Republic of China

    Xin Li, Jing He, Changle Pang & Yu Deng

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  1. Shan Liu
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  2. Xin Li
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Correspondence to Shubiao Wu.

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Liu, S., Li, X., Wu, S. et al. Fungal Pretreatment by Phanerochaete chrysosporium for Enhancement of Biogas Production from Corn Stover Silage. Appl Biochem Biotechnol 174, 1907–1918 (2014). https://doi.org/10.1007/s12010-014-1185-7

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  • DOI: https://doi.org/10.1007/s12010-014-1185-7

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