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l-Ribose Production from l-Arabinose by Immobilized Recombinant Escherichia coli Co-expressing the l-Arabinose Isomerase and Mannose-6-Phosphate Isomerase Genes from Geobacillus thermodenitrificans

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Abstract

l-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the l-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The immobilized cells produced 99 g/l l-ribose from 300 g/l l-arabinose in 3 h at pH 7.5 and 60 °C in the presence of 1 mM Co2+, with a conversion yield of 33 % (w/w) and a productivity of 33 g/l/h. The immobilized cells in the packed-bed bioreactor at a dilution rate of 0.2 h−1 produced an average of 100 g/l l-ribose with a conversion yield of 33 % and a productivity of 5.0 g/l/h for the first 12 days, and the operational half-life in the bioreactor was 28 days. Our study is first verification for l-ribose production by long-term operation and feasible for cost-effective commercialization. The immobilized cells in the present study also showed the highest conversion yield among processes from l-arabinose as the substrate.

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Acknowledgments

This work was supported by the Basic Research Lab program (No. 2010–0019306), the National Research Foundation, the Ministry of Education, Science and Technology, Republic of Korea.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, Republic of Korea

    Kyoung-Rok Kim, Eun-Sun Seo & Deok-Kun Oh

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  1. Kyoung-Rok Kim
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  2. Eun-Sun Seo
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  3. Deok-Kun Oh
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Correspondence to Deok-Kun Oh.

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Kim, KR., Seo, ES. & Oh, DK. l-Ribose Production from l-Arabinose by Immobilized Recombinant Escherichia coli Co-expressing the l-Arabinose Isomerase and Mannose-6-Phosphate Isomerase Genes from Geobacillus thermodenitrificans . Appl Biochem Biotechnol 172, 275–288 (2014). https://doi.org/10.1007/s12010-013-0547-x

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  • DOI: https://doi.org/10.1007/s12010-013-0547-x

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