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Linking Performance with Microbial Community Characteristics in an Anaerobic Baffled Reactor

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Abstract

The performance and microbial community characteristics of a laboratory scale anaerobic baffled reactor (ABR) with four compartments (C1–C4) treating sugar refinery wastewater were investigated. The COD removal was 94.8 % with a CH4 yield of 0.21 L g−1 CODremoved at total organic loading rate (OLR) of 5.33 kg COD/m−3 day−1. Fermentative bacteria were dominant in C1 and C2, while syntrophic acetogens and methanogens were dominant in C3 and C4. Some acid-tolerant methanogens were enriched in acidogenic phase. The present of the acid-tolerant methanogens could improve the efficiency and stability of the ABR as the most of the methanogens are vulnerable to low pH. In addition, high functional redundancy of the fermentative bacteria implicated that the microbial communities in acidogenic phase were stable functionally and allowed the ABR to balance perturbation. It was also found that syntrophic acetogenesis might be a weakness in the ABR as syntrophic acetogens were poor as compared with fermentative bacteria and methanogens.

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Abbreviations

ABR:

Anaerobic baffled reactor

COD:

Chemical oxygen demand

PCR-DGGE:

Polymerase chain reaction-denaturing gradient gel electrophoresis

VFA:

Volatile fatty acid

HRT:

Hydraulic retention time

OLR:

Organic loading rate.

ORP:

Oxidation-reduction potential

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 51178136) and State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. 2010DX06).

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China

    Qiaoying Ban, Jianzheng Li, Liguo Zhang, Ajay Kumar Jha & Loring Nies

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  1. Qiaoying Ban
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  2. Jianzheng Li
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  3. Liguo Zhang
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Correspondence to Jianzheng Li.

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Ban, Q., Li, J., Zhang, L. et al. Linking Performance with Microbial Community Characteristics in an Anaerobic Baffled Reactor. Appl Biochem Biotechnol 169, 1822–1836 (2013). https://doi.org/10.1007/s12010-013-0105-6

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  • DOI: https://doi.org/10.1007/s12010-013-0105-6

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