Skip to main content
SpringerLink
Log in

Kinetic Modeling of Fermentative Production of 1, 3-Propanediol by Klebsiella pneumoniae HR526 with Consideration of Multiple Product Inhibitions

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

During the fermentative production of 1, 3-propanediol (1,3-PD), the multiple product inhibitions cannot be negligible to accurately describe the kinetics of fermentation process. A kinetic model for fermentative production of 1,3-PD by Klebsiella pneumoniae HR526 with glycerol as carbon source under aerobic condition was proposed. The inhibitions of multiple products including 1,3-PD, 2, 3-butanediol (2,3-BD), acetate, and succinate were considered in the model. It was found that 1,3-PD, 2,3-BD, and acetate showed strong inhibitions to cell growth depending on their concentrations. The kinetic model was relatively accurate to predict the experimental data of batch, fed-batch, and continuous fermentations. The model thus can serve as a tool for further controlling and optimizing the fermentation process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

X :

Cell biomass concentration (g⋅L−1)

P :

1,3-PD concentration (g⋅L−1)

P B :

2,3-BD concentration (g⋅L−1)

P A :

Acetate concentration (g⋅L−1)

P S :

Succinate concentration (g⋅L−1)

S :

Glycerol concentration (g⋅L−1)

μ m :

Maximum specific growth rate (h−1)

K S :

Saturation constant for cell growth rate (g⋅L−1)

α :

Kinetic constant for 1,3-PD formation from cell growth (g⋅g−1)

β :

Kinetic constant for 1,3-PD formation from biomass (h−1)

α B :

Kinetic constant for 2,3-BD formation from cell growth (g⋅g−1)

β B :

Kinetic constant for 2,3-BD formation from biomass (h−1)

α A :

Kinetic constant for acetate formation from cell growth (g⋅g−1)

β A :

Kinetic constant for acetate formation from biomass (h−1)

α S :

Kinetic constant for succinate formation from cell growth (g⋅g−1)

β S :

Kinetic constant for succinate formation from biomass (h−1)

Y X/S :

Yield coefficient of biomass on glycerol (g⋅g−1)

Y P/S :

Yield coefficient of 1,3-PD on glycerol (g⋅g−1)

Y PB/S :

Yield coefficient of 2,3-BD on glycerol (g⋅g−1)

Y PA/S :

Yield coefficient of acetate on glycerol (g⋅g−1)

Y PS/S :

Yield coefficient of succinate on glycerol (g⋅g−1)

n :

Kinetic constant for glycerol consumption on cell metabolism (h−1)

\( C_P^{*} \) :

Critical inhibition concentration for 1,3-PD of maximum specific growth rate (g⋅L−1)

\( C_B^{*} \) :

Critical inhibition concentration for 2,3-BD of maximum specific growth rate (g⋅L−1)

\( C_A^{*} \) :

Critical inhibition concentration for acetate of maximum specific growth rate (g⋅L−1)

P*:

Critical feedback inhibition concentration of 1,3-PD for 1,3-PD formation (g⋅L−1)

\( P_B^{*} \) :

Critical feedback inhibition concentration of 2,3-BD for 2,3-BD formation (g⋅L−1)

\( P_A^{*} \) :

Critical feedback inhibition concentration of acetate for acetate formation (g⋅L−1)

\( P_S^{*} \) :

Critical feedback inhibition concentration of succinate for succinate formation (g⋅L−1)

References

  1. Huang, H., Gong, C. C., & Tsao, G. T. (2002). Production of 1, 3-propanediol by Klebsiella pneumoniae. Applied Biochemistry and Biotechnology, 98, 687–698.

    Article  Google Scholar 

  2. Zeng, A. P., & Biebl, H. (2002). Bulk chemicals from biotechnology: the case of 1, 3-propanediol production and the new trends. Advances in Biochemical Engineering/Biotechnology, 74, 239–259.

    Article  CAS  Google Scholar 

  3. Biebl, H., Menzel, K., Zeng, A. P., & Deckwer, W. D. (1999). Microbial production of 1, 3-propanediol. Applied Microbiology and Biotechnology, 52, 289–297.

    Article  CAS  Google Scholar 

  4. Arntz, D., Haas, T., Müller, A., & Wiegand, N. (1991). Kinetische untersuchung zur hydrat isierung von acrolein. Chem. Ing. Tech., 63, 733–735.

    Article  CAS  Google Scholar 

  5. Besson, M., Gallezot, P., Pigamo, A., & Reifsnyder, S. (2003). Development of an improved continuous hydrogenation process for the production of 1, 3-propanediol using titania supported ruthenium catalysts. Appl. Catal. A, 250, 117–124.

    Article  CAS  Google Scholar 

  6. Knifton, J. F., James, T. G., Slaugh, L. H., Allen, K. D., Weider, P. R. and Powell J. B. (2004). One-step production of 1, 3-propanediol from ethylene oxide and syngas with a cobalt-iron catalyst. US Patent 6.750.373.

  7. Biebl, H., & Marten, S. (1995). Fermentation of glycerol to 1, 3-propanediol: use of cosubstrates. Applied Microbiology and Biotechnology, 44, 15–19.

    Article  CAS  Google Scholar 

  8. Decker, W. D. (1995). Microbial conversion of glycerol to 1, 3-propanediol, in International Congress on Beyond 2000—Chemicals from Biotechnology: Ecological Challenge and Economic Restraints, Proc. FEMS Microbiology Reviews, 16, 143–149.

    Article  Google Scholar 

  9. Xu, Y. Z., Liu, H. J., Du, W., Sun, Y., Ou, X. J., & Liu, D. H. (2009). Integrated production for biodiesel and 1, 3-propanediol with lipase-catalyzed transesterification and fermentation. Biotechnol. Let., 31, 1335–1341.

    Article  CAS  Google Scholar 

  10. Charles, E. N., & Gregory, M. W. (2003). Metabolic engineering for the microbial production of 1, 3-propanediol. Current Opinion in Biotechnology, 14, 454–459.

    Article  Google Scholar 

  11. Zheng, Z. M., Xu, Y. Z., Liu, H. J., Guo, N. N., Cai, Z. Z., & Liu, D. H. (2008). Physiologic mechanisms of sequential products synthesis in 1, 3-propanediol fed-batch fermentation by Klebsiella pneumoniae. Biotechnology and Bioengineering, 100, 923–932.

    Article  CAS  Google Scholar 

  12. Chen, Z., Liu, H. J., Zhang, J. A., & Liu, D. H. (2009). Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions. Process Biochemistry, 44, 862–868.

    Article  CAS  Google Scholar 

  13. Xu, Y. Z., Guo, N. N., Zheng, Z. M., Ou, X. J., Liu, H. J., & Liu, D. H. (2009). Metabolism in 1, 3-propanediol fed-batch fermentation by a D-lactate deficient mutant of Klebsiella pneumoniae. Biotechnology and Bioengineering, 104, 965–972.

    Article  CAS  Google Scholar 

  14. Chen, Z., Liu, H. J., & Liu, D. H. (2011). Metabolic pathway analysis of 1, 3-propanediol production with a genetically modified Klebsiella pneumoniae by overexpressing an endogenous NADPH-dependent alcohol dehydrogenase. Biochemical Engineering Journal, 54, 151–157.

    Article  CAS  Google Scholar 

  15. Cheng, K. K., Liu, D. H., Sun, Y., & Liu, W. B. (2004). 1, 3-Propanediol production by Klebsiella pneumoniae under different aeration strategies. Biotechnology Letters, 26, 911–915.

    Article  CAS  Google Scholar 

  16. Zheng, Z. M., Hu, Q. L., Hao, J., Xu, F., Guo, N. N., Sun, Y., et al. (2008). Statistical optimization of culture conditions for 1, 3-propanediol by Klebsiella pneumoniae AC 15 via central composite design. Bioresource Technology, 99, 1052–1056.

    Article  CAS  Google Scholar 

  17. Zheng, Z. M., Guo, N. N., Hao, J., Cheng, K. K., Sun, Y., & Liu, D. H. (2009). Scale-up of micro-aerobic 1, 3-propanediol production with Klebsiella pneumonia CGMCC 1.6366. Process Biochemistry, 44, 944–948.

    Article  CAS  Google Scholar 

  18. Zheng, Z. M., Xu, Y. Z., Wang, T. P., Dong, C. Q., Yang, Y. P., & Liu, D. H. (2010). Ammonium and phosphate limitation in 1, 3-propanediol production by Klebsiella pneumoniae. Biotechnology Letters, 32, 289–294.

    Article  CAS  Google Scholar 

  19. Zeng, A. P., Ross, A., Biebl, H., Tag, C., Günzel, B., & Deckwer, W. D. (1994). Multiple product inhibition and growth modeling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation. Biotechnology and Bioengineering, 44, 902–911.

    Article  CAS  Google Scholar 

  20. Zeng, A. P. (1995). A kinetic model for product formation of microbial and mammalian cells. Biotechnology and Bioengineering, 46, 314–324.

    Article  CAS  Google Scholar 

  21. Zeng, A. P., & Deckwer, W. D. (1995). A kinetic model for substrate and energy consumption of microbial growth under substrate-sufficient conditions. Biotechnol. Progr., 11, 71–79.

    Article  CAS  Google Scholar 

  22. Chen, Z., Zheng, Z. M., Sun, Y., Hong, A. A., Peng, F., Liu, C. M., et al. (2009). Fermentation characteristics of the fast conversion of glycerol to 1, 3-propanediol by Klebsiella pneumoniae HR526. Microbiology, 36, 799–803.

    CAS  Google Scholar 

  23. Liu, H. J., Xu, Y. Z., Zheng, Z. M., & Liu, D. H. (2010). 1, 3-Propanediol and its copolymers: research, development and industrialization. Biotechnology Journal, 5, 1137–1148.

    Article  CAS  Google Scholar 

  24. Xiu, Z. L., Zeng, A. P., & An, L. J. (2000). Mathematical modeling of kinetics and research on multiplicity of glycerol bioconversion to 1, 3-propanediol. J Dalian Univ Technol, 40, 428–433.

    CAS  Google Scholar 

  25. Hao, J., Lin, R. H., Zheng, Z. M., Liu, H. J., & Liu, D. H. (2008). Isolation and characterization of microorganisms able to produce 1, 3-propanediol under aerobic conditions. World Journal of Microbiology and Biotechnology, 24, 1731–1740.

    Article  CAS  Google Scholar 

  26. Cheng, K. K., Liu, H. J., & Liu, D. H. (2005). Multiple growth inhibition of Klebsiella pneumoniae in 1, 3-propanediol fermentation. Biotechnology Letters, 27, 19–22.

    Article  CAS  Google Scholar 

  27. Zheng, Z. M., Cheng, K. K., Hu, Q. L., Liu, H. J., Guo, N. N., & Liu, D. H. (2008). Effect of culture conditions on 3-hydroxypropionaldehyde detoxification in 1, 3-propanediol fermentation by Klebsiella pneumoniae. Biochemical Engineering Journal, 39, 305–310.

    Article  CAS  Google Scholar 

  28. Hao, J., Lin, R. H., Zheng, Z. M., Sun, Y., & Liu, D. H. (2008). 3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1, 3-propanediol production by Klebsiella pneumoniae. Journal of Industrial Microbiology and Biotechnology, 35, 1615–1624.

    Article  CAS  Google Scholar 

  29. Chen, Z., Liu, H. J., & Liu, D. H. (2009). Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes. Enzyme and Microbial Technology, 45, 305–309.

    Article  Google Scholar 

  30. Cheng, K. K., Lin, R. H., Liu, H. J., & Liu, D. H. (2005). Kinetic analysis of anaerobic fermentation of 1, 3-propanediol by Klebsiella pneumoniae. The Chinese Journal of Process Engineering, 5, 425–429.

    CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the International Collaboration Project of the Ministry of Science and Technology of China (no. 2010DFB40170) for supporting this work.

Author information

Authors and Affiliations

  1. Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    Lu He, Xuebing Zhao, Yan Sun & Dehua Liu

  2. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China

    Keke Cheng

Authors
  1. Lu He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xuebing Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Keke Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dehua Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xuebing Zhao or Dehua Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, L., Zhao, X., Cheng, K. et al. Kinetic Modeling of Fermentative Production of 1, 3-Propanediol by Klebsiella pneumoniae HR526 with Consideration of Multiple Product Inhibitions. Appl Biochem Biotechnol 169, 312–326 (2013). https://doi.org/10.1007/s12010-012-9984-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9984-1

Keywords

Search

Navigation