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Metabolomic Profilings of Urine and Serum from High Fat-Fed Rats via 1H NMR Spectroscopy and Pattern Recognition

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Abstract

1H NMR spectroscopy in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine and serum of high fat-fed rats relative to normal chow-fed ones. Metabolites contributing to intergroup discrimination identified by partial least squares discriminant analysis include 3-hydroxybutyrate, glutamate, glutamine, citrate, choline, hippurate, alanine, lactate, creatinine, taurine, acetate, etc. The aging effect along with long-term feeding was delineated with metabolic trajectory in principal component analysis score plot and age-related differences on metabolic profiling under different dietary intervention were recognised. The identified metabolites responsible for obesity were all imported into a web tool for network-based interpretation of compound lists to interpret their functional context, molecular mechanisms and disturbed signalling pathway globally and systematically. The results are useful for interpreting the pathology of obesity and further probing into the relationship between dietary-induced obesity and type 2 diabetes mellitus.

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Acknowledgments

This work was supported by the NNSF of China (11074209, 81201143 and 81073113), the Fundamental Research Funds for the Central Universities (2011121046), the Excellent Youth Foundation of Fujian Province (2009D016) and Xiamen Research Platform on Systems Biology of Metabolic Diseases.

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Authors and Affiliations

  1. Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, 361005, China

    Jingjing Xu, Shuhui Cai, Jiyang Dong, Jianghua Feng & Zhong Chen

  2. The First Affiliated Hospital of Xiamen University, The Xiamen Diabetes Institute, Xiamen, 361001, China

    Changqin Liu & Xuejun Li

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  1. Jingjing Xu
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  2. Changqin Liu
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  3. Shuhui Cai
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  4. Jiyang Dong
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  6. Jianghua Feng
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  7. Zhong Chen
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Correspondence to Shuhui Cai or Xuejun Li.

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Xu, J., Liu, C., Cai, S. et al. Metabolomic Profilings of Urine and Serum from High Fat-Fed Rats via 1H NMR Spectroscopy and Pattern Recognition. Appl Biochem Biotechnol 169, 1250–1261 (2013). https://doi.org/10.1007/s12010-012-0072-3

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