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Continuous Ethanol Production from Cassava Through Simultaneous Saccharification and Fermentation by Self-Flocculating Yeast Saccharomyces Cerevisiae CHFY0321

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Abstract

In this study, a fermentor consisting of four linked stirred towers that can be used for simultaneous saccharification and fermentation (SSF) and for the accumulation of cell mass was applied to the continuous production of ethanol using cassava as the starchy material. For the continuous process with SSF, the pretreated cassava liquor and saccharification enzyme at total sugar concentrations of 175 g/L and 195 g/L were continuously fed to the fermentor with dilution rates of 0.014, 0.021, 0.031, 0.042, and 0.05 h−1. Considering the maximum saccharification time, the highest volumetric productivity and ethanol yield were observed at a dilution rate of 0.042 h−1. At dilution rates in the range of 0.014 h−1 to 0.042 h−1, high production rates were observed, and the yeast in the first to fourth fermentor showed long-term stability for 2 months with good performance. Under the optimal culture conditions with a feed sugar concentration of 195 g/L and dilution rate of 0.042 h−1, the ethanol volumetric productivity and ethanol yield were 3.58 g/L∙h and 86.2%, respectively. The cell concentrations in the first to fourth stirred tower fermentors were 74.3, 71.5, 71.2, and 70.1 g dry cell/L, respectively. The self-flocculating yeast, Saccharomyces cerevisiae CHFY0321, developed by our group showed excellent fermentation results under continuous ethanol production.

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Authors and Affiliations

  1. Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Palbok-Dong 829, Dukjin-Gu, Jeonju, 561-203, Korea

    Gi-Wook Choi, Hyun-Woo Kang & Se-Kwon Moon

  2. School of Chemical Engineering, Chonbuk National University, Deokjin-Dong 664-14, Dukjin-Gu, Jeonju, 561-156, Korea

    Bong-Woo Chung

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  1. Gi-Wook Choi
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  2. Hyun-Woo Kang
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Correspondence to Gi-Wook Choi.

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Choi, GW., Kang, HW., Moon, SK. et al. Continuous Ethanol Production from Cassava Through Simultaneous Saccharification and Fermentation by Self-Flocculating Yeast Saccharomyces Cerevisiae CHFY0321. Appl Biochem Biotechnol 160, 1517–1527 (2010). https://doi.org/10.1007/s12010-009-8653-5

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  • DOI: https://doi.org/10.1007/s12010-009-8653-5

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