Abstract
Currently, natural pigments are gaining attention as an alternative to synthetic colouring agents for food and non-food related applications. To this end, a natural, red coloured, and water-soluble extracellular pigment was generated from Talaromyces purpurogenus. The colour profile of the pigment was estimated at different concentrations, and the thermal degradation kinetics were modelled using the Arrhenius, Ball, and second-order kinetic models, showing a correlation between the rate of pigment degradation and an increase in temperature. The half-life of the pigment decreased as the temperature was increased from 30 to 90 °C, and the kinetics were adequately represented by the studied models. The pigment was more susceptible to degradation at lower pH values, exhibiting an inverse correlation between rate of decay and pH values, for thermal treatment at 70 and 90 °C. In the studied pH range, the maximum half-life and decimal reduction time were observed at pH 8. The non-thermal degradation was ultraviolet (UV)-dosage dependent, with half-lives decreasing with increase in intensity of radiation. For UV-A degradation kinetics, the rate constant (k) values obtained using the first-order and Weibull kinetic models were consistent, whereas such k values differed for UV-C irradiation. An extracellular pigment–E. coli model was used to ascertain the effect of pigment concentration on UV sterilisation. The photoprotective effect was found to be concentration dependent, with time required for half-cell death increasing with an increase in pigment concentration. The pigment was found to be compatible with commonly used thermal and non-thermal processing conditions.
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Abbreviations
- CFU :
-
Colony forming units
- LB :
-
Luria–Bertani
- MSE :
-
Mean square error
- U.O.D. :
-
Units of optical density
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This work was partially supported by JSPS KAKENHI Grant-in Aid for Scientific Research B (19H03086), the Sumitomo Electric Industries Group Corporate Social Responsibility Foundation, Takahashi Industrial and Economic Research Foundation [10–003-183, 11–003-016], and Grant for Environmental Research Project from the Sumitomo Foundation (grant to Hideki Aoyagi).
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S. B. and H. A. designed the experiments and S. B. performed them. H. A. supervised the research. S. B. and H. A. analysed and interpreted the results and wrote the manuscript. Both authors read and approved the final manuscript.
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Bhatnagar, S., Aoyagi, H. Thermal and UV Degradation Kinetics of Water-Soluble Extracellular Pigment Produced by Talaromyces purpurogenus. Food Bioprocess Technol 15, 606–619 (2022). https://doi.org/10.1007/s11947-021-02733-9
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DOI: https://doi.org/10.1007/s11947-021-02733-9