Generation of Linear and Parabolic Concentration Gradients by Using a Christmas Tree-Shaped Microfluidic Network

  • Qilong Shen
  • Qiongwei Zhou
  • Zhigang Lu
  • Nangang Zhang
Engineering Science


This paper describes a simple method of generating concentration gradients with linear and parabolic profiles by using a Christmas tree-shaped microfluidic network. The microfluidic gradient generator consists of two parts: a Christmas tree-shaped network for gradient generation and a broad microchannel for detection. A two-dimensional model was built to analyze the flow field and the mass transfer in the microfluidic network. The simulating results show that a series of linear and parabolic gradient profiles were generated via adjusting relative flow rate ratios of the two source solutions (RL2 ≥0.995 and RP2 ≥0.999), which could match well with the experimental results (RL2 ≥0.987 and RP2 ≥0.996). The proposed method is promising for the generation of linear and parabolic concentration gradient profiles, with the potential in chemical and biological applications such as combinatorial chemistry synthesis, stem cell differentiation or cytotoxicity assays.

Key words

tree-shaped network concentration gradient linear profile parabolic profile 

CLC number

O 351 


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Copyright information

© Wuhan University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Qilong Shen
    • 1
  • Qiongwei Zhou
    • 1
  • Zhigang Lu
    • 1
  • Nangang Zhang
    • 1
  1. 1.School of Electrical and Electronic EngineeringWuhan Textile UniversityWuhan, HubeiChina

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