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Allicin-Loaded Hydroxyapatite: Enhanced Release, Cytocompatibility, and Antibacterial Properties for Bone Tissue Engineering Applications

  • Interactions between Biomaterials and Biological Tissues and Cells
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Abstract

Allicin, the active compound of garlic extract, is a naturally sourced biomolecule, which promotes a vast range of health benefits. However, the limited stability of allicin restricts its applications in tissue engineering. Additionally, the detailed effects of allicin in bone health are yet to be explored. Our work reports on the fabrication of a novel allicin-loaded hydroxyapatite drug delivery system with enhanced biological properties. The fabricated system shows excellent antibacterial efficiency against S. aureus after 36 h of bacterial interaction with a sample. The allicin release kinetics are enhanced with polycaprolactone (PCL). The obtained results after 20 days of drug release study indicate that PCL coating leads to an increase in cumulative allicin release from ~ 35% to 70% at a physiological pH of 7.4. These scaffolds maintain stability during the whole period of drug release. Cytocompatibility of tested compositions with osteoblasts indicates enhanced cell viability and good filopodial attachment on the sample surface at day 7. These allicin-loaded antibacterial and cytocompatible scaffolds can find applications as localized delivery vehicles for bone tissue engineering.

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Acknowledgements

The authors acknowledge financial support from the National Institute of Dental and Craniofacial Research (NIDCR) of the National Institutes of Health, Grant Number R01 DE029204-01 (PI: Bose), and the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) of the National Institutes of Health under Grant Number R01 AR066361 (PI: Bose). The content is solely the authors’ responsibility and does not necessarily represent the National Institutes of Health’s official views.

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  1. W. M. Keck Biomedical Materials Research Laboratory, Washington State University, Pullman, WA, 99164, USA

    Susmita Bose, Arjak Bhattacharjee, Christine Huynh & Dishary Banerjee

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  1. Susmita Bose
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  2. Arjak Bhattacharjee
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  3. Christine Huynh
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  4. Dishary Banerjee
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Correspondence to Susmita Bose.

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Bose, S., Bhattacharjee, A., Huynh, C. et al. Allicin-Loaded Hydroxyapatite: Enhanced Release, Cytocompatibility, and Antibacterial Properties for Bone Tissue Engineering Applications. JOM 74, 3349–3356 (2022). https://doi.org/10.1007/s11837-022-05366-1

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  • DOI: https://doi.org/10.1007/s11837-022-05366-1

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