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P58IPK facilitates plant recovery from ER stress by enhancing protein synthesis

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Abstract

P58IPK has been implicated in eukaryotic ER stress responses and viral pathogenesis, however, its biological functions and molecular mechanism in plants are unclear. Prolonged ER stress produced by tunicamycin (TM) increased P58IPK mRNA and protein levels in Arabidopsis. Although the growth of 2 × 35S:P58IPK-myc plants was less severely inhibited than that of Col-0 plants, TM inhibited the growth of p58ipk-2 mutants more severely than that of Col-0 plants. Under prolonged ER stress conditions, the unfolded protein response (UPR)-related genes were expressed at a higher level in the p58ipk-2 mutants than in Col-0 plants. Protein synthesis inhibition by TM in 2 × 35S:P58IPK-myc plants was lower than in Col-0 plants under prolonged ER stress conditions, however, not significantly different in p58ipk-2 mutants. The GST-P58IPK protein exhibited both chaperone and RNA-binding activities in a dose-dependent manner. P58IPK has been shown to interact with ribosomes, allowing for enhanced protein production on the ER membrane. Following ER stress, 2 × 35S:P58IPK-myc plants recovered better than Col-0, but p58ipk-2 mutants recovered less than Col-0. These findings reveal that P58IPK can promote protein translation in association with ribosomes and contribute to stress recovery in Arabidopsis when induced during the last phase of ER stress.

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Acknowledgements

This work was supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ016236) and by the National Research Foundation of Korea (NRF, 2021R1A2C1013516, 2019R1I1A1A01058736, 2022R1I1A1A01071947, and 2020R1A6A1A03044344) Grants funded by the Korean government (Rural Development Administration, the Ministry of Science and ICT, and Ministry of Education). B.N. Vu, Y.E. Lee, H.N. Choi and Y.N. Lee were supported by the BK4 program funded by the Ministry of Education of Korea.

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  1. Ki Seong Ko and Jae Yong Yoo have contributed equally to this work and share first authorship.

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  1. Division of Life Science, Division of Applied Life Sciences (BK4 Program) and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-Daero, Jinju, 52828, Korea

    Ki Seong Ko, Jae Yong Yoo, Kyung Hwa Kim, Bo Young Hwang, Bich Ngoc Vu, Young Eun Lee, Ha Na Choi, Yoo Na Lee, Jihee Yun, Ji Ye Park, Woo Sik Chung, Jong Chan Hong & Kyun Oh Lee

  2. Chuncheon Center, Korean Basic Science Institute, Gangwon-Do Chuncheon, 24341, Korea

    Myeong Seon Jeong

  3. Department of Biochemistry, College of Natural Sciences, Kangwon National University, 1 Kangwondaehak-Gil, Chuncheon, 24341, Korea

    Hyun Suk Jung

  4. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Korea

    Su Kyoung Jung & Jeong Mee Park

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Contributions

KO Lee planned, designed and organized the research and wrote the research paper. KSK, JYY, KHK, BYH, BNV, YEL, HNC, YNL, JY, JYP, WSC and JCH performed and analyzed the experiments shown in Figs. 1–8, Supporting Information Figs. 1–4 and 6, 7 and Supporting Information Tables 1, 2. KSK and JYY contributed most extensively to the work presented in this paper. MSJ and HSJ carried out and evaluated the experiments shown in Fig. 5 and in Supporting Information Fig. 5. SKJ, JMP carried out and reviewed the studies shown in Supporting Information Fig. 8. WSC, JCH, HSJ and JMP provided assistance in the study and interpretation of the data. All authors reviewed the results and approved the final version of the manuscript.

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Ko, K.S., Yoo, J.Y., Kim, K.H. et al. P58IPK facilitates plant recovery from ER stress by enhancing protein synthesis. Plant Biotechnol Rep 16, 665–681 (2022). https://doi.org/10.1007/s11816-022-00797-3

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