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Construction of methanol sensing Escherichia coli by the introduction of novel chimeric MxcQZ/OmpR two-component system from Methylobacterium organophilum XX

  • Biotechnology
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Abstract

Methylobacterium organophilum XX is a type II facultative methylotroph that can grow on methanol. In M. organophilum XX, the MxcQ/MxcE two-component system (TCS) is involved in methanol metabolism. EnvZ/OmpR in E. coli TCS was exploited to develop a methanol biosensor by engaging the MxcQ/MxcE TCS system. The MxcQZ/OmpR methanol sensing chimeric TCS was constructed by integrating the sensing domain of M. organophilum MxcQ with the transmitter domain of E. coli EnvZ. The response regulator of the chimeric TCS system is OmpR, which regulates the expression of the ompC and gfp. The expression of ompC was monitored by real-time quantitative PCR analysis. The expression of gfp also confirmed the expression of the ompC. The maximum expression of ompC and gfp occurred with 0.05% of methanol, and the expression started to decline with further increases in methanol concentration. This system delivers rapid detection of methanol in the environment.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Namgu, Ulsan, 44610, Korea

    Vidhya Selvamani, Murali Kannan Maruthamuthu & Soon Ho Hong

  2. Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 600036, India

    Kulandaisamy Arulsamy

  3. Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), 45 Jongga-ro, Jung-gu, Ulsan, 44429, Korea

    Gyeong Tae Eom

  4. Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34144, Korea

    Gyeong Tae Eom

Authors
  1. Vidhya Selvamani
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  2. Murali Kannan Maruthamuthu
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  3. Kulandaisamy Arulsamy
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  4. Gyeong Tae Eom
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  5. Soon Ho Hong
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Correspondence to Soon Ho Hong.

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Selvamani, V., Maruthamuthu, M.K., Arulsamy, K. et al. Construction of methanol sensing Escherichia coli by the introduction of novel chimeric MxcQZ/OmpR two-component system from Methylobacterium organophilum XX. Korean J. Chem. Eng. 34, 1734–1739 (2017). https://doi.org/10.1007/s11814-017-0063-8

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  • DOI: https://doi.org/10.1007/s11814-017-0063-8

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