Skip to main content

Advertisement

SpringerLink
Log in

Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources

  • Biotechnology
  • Published:
Korean Journal of Chemical Engineering Aims and scope Submit manuscript

Abstract

Metabolite profiling was conducted to monitor the metabolic differences of the Ralstonia eutropha H16, polyhydroxybutyrate (PHB) producing strain, when grown on different sole carbon sources and to identify the effect of the deletion of the PHB production pathway. The combination of rapid filtration and boiling ethanol extraction successfully extracted intracellular metabolites. Accumulation of acetyl-CoA was detected when the strain was grown on fructose. Whereas, lower adenylate energy charge (AEC) ratio and cell mass was identified on the cells grown on acetate. No significant difference was detected between the wild type and mutant strain except metabolites in the PHB synthetic pathway. Increased PHB production is expected on fructose due to the accumulation of AcCoA, while it is more difficult to improve the production on acetate because of lower metabolic precursor level and AEC ratio.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Chanprateep, J. Biosci. Bioeng., 110, 621 (2010).

    Article  CAS  Google Scholar 

  2. G.-Q. Chen, Microbiol. Monograph., 14, 17 (2010).

    Article  Google Scholar 

  3. M. Koller, A. Salerno, A. Muhr, A. Reiterer and G. Braunegg, Mater. Technol., 47, 5 (2013).

    CAS  Google Scholar 

  4. T. Fukui, K. Chou, K. Harada, I. Orita, Y. Nakayama, T. Bamba, S. Nakamura and E. Fukusaki, Metabolomics, 10, 190 (2014).

    Article  CAS  Google Scholar 

  5. T.A. Leaf and F. Srienc, Biotechnol. Bioeng., 57, 557 (1998).

    Article  CAS  Google Scholar 

  6. B. H. A. Rehm, Biochem. J., 376, 15 (2003).

    Article  CAS  Google Scholar 

  7. C. Pena, T. Castillo, A. Garcia, M. Millan and D. Segura, Microb. Biotechnol., 7, 278 (2014).

    Article  CAS  Google Scholar 

  8. D. Segura and G. Espin, Appl. Microbiol. Biotechnol., 65, 414 (2004).

    Article  CAS  Google Scholar 

  9. R. Noguez, D. Segura, S. Moreno, A. Hernandez, K. Juarez and G. Espin, J. Mol. Microbiol. Biotechnol., 15, 244 (2008).

    Article  CAS  Google Scholar 

  10. C. Pena, S. Lopez, A. Garcia, G. Espin, A. Romo-Uribe and D. Segura, Ann. Microbiol., 64, 39 (2014).

    Article  CAS  Google Scholar 

  11. M. Akiyama, T. Tsuge and Y. Doi, Polym. Degrad. Stab., 80, 183 (2003).

    Article  CAS  Google Scholar 

  12. C. F. Budde, S. L. Riedel, F. Hubner, S. Risch, M. K. Popovic, C. Rha and A. J. Sinskey, Appl. Microbiol. Biotechnol., 89, 1611 (2011).

    Article  CAS  Google Scholar 

  13. S. L. Riedel, J. Bader, C. J. Brigham, C. F. Budde, Z. a. M. Yusof, C. Rha and A. J. Sinskey, Biotechnol. Bioeng., 109, 74 (2012).

    Article  CAS  Google Scholar 

  14. O. Fiehn, Plant Mol. Biol., 48, 155 (2002).

    Article  CAS  Google Scholar 

  15. M.R. Mashego, K. Rumbold, M. De Mey, E. Vandamme, W. Soetaert and J. J. Heijnen, Biotechnol. Lett., 29, 1 (2007).

    Article  CAS  Google Scholar 

  16. D. Vuckovic, Anal. Bioanal. Chem., 403, 1523 (2012).

    Article  CAS  Google Scholar 

  17. S. Kim, D.Y. Lee, G. Wohlgemuth, H. S. Park, O. Fiehn and K.H. Kim, Anal. Chem., 85, 2169 (2013).

    Article  CAS  Google Scholar 

  18. X.Y. Wang, F. X. Yang, Y. S. Zhang, G.W. Xu, Y. Liu, J. Tian and P. Gao, Electrophoresis, 36, 2140 (2015).

    Article  CAS  Google Scholar 

  19. H.M. Lee, B.Y. Jeon and M. K. Oh, Biotechnol. Bioproc. Eng., 21(3), 402 (2016).

    Article  CAS  Google Scholar 

  20. J.M. Buescher, S. Moco, U. Sauer and N. Zamboni, Anal. Chem., 82, 4403 (2010).

    Article  CAS  Google Scholar 

  21. M. Friedman, J. Agric. Food Chem., 52, 385 (2004).

    Article  CAS  Google Scholar 

  22. G.D. Saratale and M.K. Oh, Int. J. Biol. Macromol., 80, 627 (2015).

    Article  CAS  Google Scholar 

  23. B. Gonzalez, J. Francois and M. Renaud, Yeast, 13, 1347 (1997).

    Article  CAS  Google Scholar 

  24. N. B. S. Jensen, K.V. Jokumsen and J. Villadsen, Biotechnol. Bioeng., 63, 356 (1999).

    Article  CAS  Google Scholar 

  25. R. P. Maharjan and T. Ferenci, Anal. Biochem., 313, 145 (2003).

    Article  Google Scholar 

  26. B. Álvarez-Sánchez, F. Priego-Capote and M.D. Luque de Castro, Trends Analyt. Chem., 29, 120 (2010).

    Article  Google Scholar 

  27. C. L. Winder, W.B. Dunn, S. Schuler, D. Broadhurst, R. Jarvis, G.M. Stephens and R. Goodacre, Anal. Chem., 80, 2939 (2008).

    Article  CAS  Google Scholar 

  28. R.D. Douma, L. P. De Jonge, C.T. H. Jonker, R. M. Seifar, J. J. Heijnen and W. M. Van Gulik, Biotechnol. Bioengin., 107, 105 (2010).

    Article  CAS  Google Scholar 

  29. L.P. De Jonge, R.D. Douma, J. J. Heijnen and W.M. Van Gulik, Metabolomics, 8, 727 (2012).

    Article  CAS  Google Scholar 

  30. W.M. Van Gulik, Curr. Opin. Biotechnol., 21, 27 (2010).

    Article  CAS  Google Scholar 

  31. C. Wittmann, J.O. Kromer, P. Kiefer, T. Binz and E. Heinzle, Anal. Biochem., 327, 135 (2004).

    Article  CAS  Google Scholar 

  32. G.D. Tredwell, B. Edwards-Jones, D. J. Leak and J.G. Bundy, Plos One, 6 (2011).

  33. A. Pohlmann, W. F. Fricke, F. Reinecke, B. Kusian, H. Liesegang, R. Cramm, T. Eitinger, C. Ewering, M. Potter, E. Schwartz, A. Strittmatter, I. Voss, G. Gottschalk, A. Steinbuchel, B. Friedrich and B. Bowien, Nat. Biotechnol., 25, 478 (2007).

    Article  CAS  Google Scholar 

  34. Y. Jian and Y. Si, Biotechnol. Prog., 20, 1015 (2004).

    Article  Google Scholar 

  35. G. Gottschalk, H. G. Schlegel and U. Eberhardt, Arch. Microbiol., 48, 95 (1964).

    CAS  Google Scholar 

  36. R. Gerstmeir, V. F. Wendisch, S. Schnicke, H. Ruan, M. Farwick, D. Reinscheid and B. J. Eikmanns, J. Biotechnol., 104, 99 (2003).

    Article  CAS  Google Scholar 

  37. M.K. Oh, L. Rohlin, K. C. Kao and J. C. Liao, J. Biol. Chem., 277, 13175 (2002).

    Article  CAS  Google Scholar 

  38. A.G. Chapman, L. Fall and D. E. Atkinson, J. Bacteriol., 108, 1072 (1971).

    CAS  Google Scholar 

  39. Z.-X. Wang, C.O. Brämer and A. Steinbüchel, FEMS Microbiol. Lett., 228, 63 (2003).

    Article  CAS  Google Scholar 

  40. H. L. Kornberg and H.A. Krebs, Nature, 179, 988 (1957).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Korea

    Dae-Kyun Im & Min-Kyu Oh

  2. CJ Research Institute of Biotechnology, Suwon, 16495, Korea

    Seok Hun Yun & Joon-Young Jung

  3. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea

    Seok Hun Yun & Jinwon Lee

Authors
  1. Dae-Kyun Im
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seok Hun Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joon-Young Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jinwon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min-Kyu Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jinwon Lee or Min-Kyu Oh.

Additional information

This article is dedicated to Prof. Ki-Pung Yoo on the occasion of his retirement from Sogang University.

Both authors contributed equally to this manuscript.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Im, DK., Yun, S.H., Jung, JY. et al. Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources. Korean J. Chem. Eng. 34, 797–805 (2017). https://doi.org/10.1007/s11814-016-0333-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11814-016-0333-x

Keywords

Search

Navigation