Abstract
Narrow genetic base in Indian lentil genotypes serves as a bottleneck in the quest for breeding improved cultivars. In this study, 43 lentil genotypes were analyzed using newly developed simple sequence repeat (SSR) markers to assess genetic diversity and population structure. Twenty polymorphic SSRs yielded a total of 71 alleles with an average of 3.55 alleles per locus. The average gene diversity was 0.51 and polymorphic information content ranged from 0.11 (Lc_Mcu6) to 0.86 (Lc_Mcu30) with an average value of 0.45. Lc_MCu30, Lc_MCu19, Lc_MCu45a and Lc_MCu12b were the most informative and discriminative SSRs. The Jaccard similarity coefficient between all pairwise genotypes ranged from 0.436 to 1. Cluster analysis discriminated the genotypes into six clusters of contrasting sizes. Maximum genetic difference (56.4%) was found between Pant L-8 and LL 1373 and, thus, these genotypes showed highest level of diversity. First three principal coordinates accounted for 51.73% of total variation. Population structure revealed four subpopulations with low genetic differentiation as evident by high admixture. Overall, low genetic variation was observed. Reasons for such results might be due to breeding programs as improved elite breeding lines such as Precoz were constantly used as parents in crosses to generate segregating populations, exhibiting limited gene flow and diversity. These findings imply that the genotypes under investigation have narrow genetic base. Thus, there is a need to introgress superior genes from diverse material, macrosperma type or related wild species to broaden the genetic base of Indian lentil genotypes.
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Sharma, R., Chaudhary, L. & Kumar, M. Microsatellites based assessment of genetic diversity and population structure of indian lentil (Lens culinaris Medik.) genotypes. Biologia 78, 2317–2328 (2023). https://doi.org/10.1007/s11756-023-01385-8
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DOI: https://doi.org/10.1007/s11756-023-01385-8