Summary
Thirty accessions of domesticated (Lens culinaris ssp. culinaris) and wild (L. culinaris ssp. orientalis, L. culinaris ssp. odemensis, L. nigricans ssp. ervoides and L. nigricans ssp. nigricans) lentil were evaluated for restriction fragment length polymorphisms (RFLPs) using ten relative low-copy-number probes selected from partial genomic and cDNA libraries of lentil. Nei's average gene diversity was used as a measure of genetic variability for restriction fragment lengths within subspecies and a dendrogram was constructed from genetic distance estimates between subspecies. The wild lentils L. culinaris ssp. orientalis and L. culinaris ssp. odemensis showed the greatest variability for restriction fragment lengths and were closely positioned to domesticated lentil in the dendrogram. Little variability for restriction fragment lengths was observed within accessions of L. nigricans ssp. ervoides and L. nigricans ssp. nigricans. This observation is consistent with a previously published proposal that nigricans may have been independently domesticated. Estimates of genetic variability based on RFLPs tended to be greater than estimates from isozymes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beckmann J, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67:35–43
Cohen D, Ladizinsky G, Meira Z, Muehlbauer F (1984) Rescue of interspecific Lens hybrids by means of embryo culture. Plant Cell Tiss Org Cult 3:343–347
Flavell R, O'Dell M, Sharp P, Nevo E, Beiles A (1986) Variation in the intergenic spacer of ribosomal DNA of wild wheat, Triticum dicoccoides, in Israel. Mol Biol Evol 3:547–558
Gottlieb L (1981) Electrophoretic evidence and plant populations. Prog Phytochem 7:1–46
Havey M, Muehlbauer F (1989) Linkages between restriction fragment length, isozyme, and morphological markers in lentil. Theor Appl Genet 77:395–401
Hoffman D, Soltis D, Muehlbauer F, Ladizinsky G (1986) Isozyme polymorphism in Lens (Leguminosae). Syst Bot 11:392–402
Ladizinsky G (1979) Species relationships in the genus Lens as indicated by seed-protein electrophoresis. Bot Gaz 140:449–451
Ladizinsky G, Braun D, Muehlbauer F (1983) Evidence for domestication of Lens nigricans (M. Bieb.) Godron in S. Europe. Bot J Linn Soc 87:169–176
Ladizinsky G, Braun D, Goshen D, Muehlbauer F (1984) The biological species of the genus Lens. Bot Gaz 145:253–261
Ladizinsky G, Cohen D, Muehlbauer F (1985) Hybridization in the genus Lens by means of embryo culture. Theor Appl Genet 70:97–101
Muehlbauer F, Slinkard A (1981) Genetics and breeding methodology. In: Webb C, Hawtin G (eds) Lentils. Commonwealth Agricultural Bureaux, London, pp 69–90
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York, 512 pp
Palmer J, Shields C, Cohen D, Orton T (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Pinkas R, Zamir D, Ladizinsky G (1985) Allozyme divergence and evolution in the genus Lens. Plant Syst Evol 151:131–150
Solh M, Erskine W (1981) Genetic resources. In: Webb C, Hawtin G (eds) Lentils. Commonwealth Agricultural Bureaux, London, pp 53–68
Soller M, Beckmann J (1983) Genetic polymorphism in varietal identification and genetic improvement. Theor Appl Genet 67:25–33
Song K, Osborn T, Williams P (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 75:784–794
Sytsma K, Gottlieb L (1986) Chloroplast DNA evidence for the origin of the genus Heterogaura from a species of Clarkia (Onagraceae). Proc Natl Acad Sci USA 83:5554–5557
Sytsma K, Schaal B (1985) Phylogenetics of the Lisianthius Skinneri (Gentianaceae) species complex in Panama utilizing DNA restriction fragment analysis. Evolution 39:594–608
Williams J, Sanchez A, Jackson M (1974) Studies on lentils and their variation. I. The taxonomy of the species. SABRAO J 6:133–145
Zamir D, Tadmor V (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147:355–358
Author information
Authors and Affiliations
Additional information
Communicated by J. Mac Key
Rights and permissions
About this article
Cite this article
Havey, M.J., Muehlbauer, F.J. Variability for restriction fragment lengths and phylogenies in lentil. Theoret. Appl. Genetics 77, 839–843 (1989). https://doi.org/10.1007/BF00268336
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00268336