Abstract
Plants harbor diverse and taxonomically structured communities of protective microorganisms that confer beneficial characteristics in plant growth and protection against biotic and abiotic stresses. The aim of this study is to characterize the composition of endophytic bacterial communities in leaves and rhizobacterial communities of the flood affected and unaffected apricot trees by 16S rRNA genes metabarcoding. Results showed a slight bacterial diversity reduction in flood affected condition compared to the unaffected one. The relative bacterial abundance analysis showed that the bacterial taxa were almost uniformly distributed between the leaves. However, a clear differential taxa abundance was observed in rhizosphere soil which may be associated with apricot tree environmental status. In fact, the flood affected rhizosphere soil showed a significant increase in several bacterial taxa including particularly Bacillus frigoritolerans and Pseudoarthrobacter phenanthrenivorans which are known to be beneficial to soil. However, some Actinobacteria genera that promote growth of plants, including Nocardioides sp., Streptomyces sp., Blastococcus and Geodermatophilus were decreased in abundance. This distribution of abundance and diversity of the bacterial community in the flood affected tree can be correlated to abiotic stress. These results provide new insights into rhizosphere-associated microbes that are likely playing a crucial role in plant growth and survival.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ASV:
-
Amplicon sequence variants
- CTAB:
-
Cetyl-trimethy ammonium bromide
- HTS:
-
High–throughput sequencing
- ICP-OES:
-
Inductive Coupled Plasma Optical Emission Spectrometry
- PGPR:
-
Plant growth-promoting rhizobacteria
- TC:
-
Total carbon
- TN:
-
Total nitrogen
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Authors would like to thank Pr. HaÏthem Sghaier for his help and advices in physico-chemical analysis of rhizosphere soil.
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The research was supported by the Tunisian Ministry of High Education and Scientific Research (LR99ES12).
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“Conceptualization, H.B., N.T.-F., J.-M.A. and M.M.-.K.; methodology, H.B., S.Z.-A., M.M.-K.; software, C.N. and F.Ar.; resources, F.Am.; writing—original draft preparation, H.B.; writing—review and editing, H.B., F.Ar., M.M.-K. All authors have read and agreed to the published version of the manuscript.”
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Bourguiba, H., Naccache, C., Zehdi-Azouzi, S. et al. Exploration of bacterial diversity in leaves and rhizosphere soil of flood affected and unaffected apricot trees. Biologia 78, 217–227 (2023). https://doi.org/10.1007/s11756-022-01228-y
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DOI: https://doi.org/10.1007/s11756-022-01228-y