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Reclassification of Brevibacterium frigoritolerans DSM 8801T as Bacillus frigoritolerans comb. nov. Based on Genome Analysis

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Abstract

16S rRNA gene sequence analysis showed that the type strain Brevibacterium frigoritolerans DSM 8801T had the highest similarity (99.7%) with the reference strain Bacillus simplex NBRC 15720T, followed by Bacillus muralis DSM 16288T (99.6%), Bacillus butanolivorans DSM 18926T (99.5%), and Bacillus loiseleuriae FJAT-27997T (97.9%). This relationship is confirmed by the phylogenetic analysis indicating that Bre. frigoritolerans DSM 8801T fell in the genus Bacillus group and formed a clade with the closely related Bacillus species. Average nucleotide identity and in silico DNA–DNA hybridization (isDDH) values between strain DSM 8801T and the most closed reference strain Bac. simplex NBRC 15720T were both much lower than species definition threshold values of 95% and 70%, respectively, which indicated strain DSM 8801T should not be affiliated to one of the validly named Bacillus species. The percentage of conserved protein (POCP) values between the strain DSM 8801T and the type strains of the above species were 80.7%, 69.2%, 72.2%, 53.6%, and 50.0%, respectively, higher than the genus definition threshold value of 50%. The main isoprenoid quinone of strain DSM 8801T was MK-7, the main polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidyl ethanolamine (PE), and the major fatty acids were anteiso-C15:0 and iso-C15:0. On the basis of the phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, Bre. frigoritolerans DSM 8801T should belong to the genus Bacillus, and be proposed to reclassify as Bacillus frigoritolerans comb. nov., with the type strain DSM 8801T (=ATCC 25097T  = CCUG 43489T  = CIP 67.20T  = JCM 11681T).

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Abbreviations

isDDH:

In silico DNA–DNA hybridization

ANI:

Average nucleotide identity

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidyl ethanolamine

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Acknowledgements

This work was financially supported by the External cooperative project of Fujian Academy of Agricultural Sciences (Grant No. DEC201821209), the Fujian Special Fund for Public Interest Research (Grant No. 2018R1017-1), the Natural Science Foundation of Fujian Province, China (Grant No. 2017J01048), the Youth Talent Plan of Fujian Academy of Agricultural Sciences (Grant No. YC2015-17) for the financial supporting, respectively.

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  1. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China

    Guo-Hong Liu, Bo Liu, Jie-Ping Wang, Jian-Mei Che & Peng-Fei Li

  2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Peng-Fei Li

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Liu, GH., Liu, B., Wang, JP. et al. Reclassification of Brevibacterium frigoritolerans DSM 8801T as Bacillus frigoritolerans comb. nov. Based on Genome Analysis. Curr Microbiol 77, 1916–1923 (2020). https://doi.org/10.1007/s00284-020-01964-x

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