Abstract
Camellia petelotii (Merr.) Sealy is a rare medicinal and edible plant in China whose wild species have a narrow distribution range and poor adaptability to strong light. To explore the light-response mechanism, the transcriptome-and photosynthesis-related physical and chemical indicators of leaves were analyzed under gradient light intensity of three processing conditions (100, 300, 700 µmol·m−2·s−1, photosynthetically active radiation, PAR) with 5 h of treatment in this study. A preliminary comparative analysis found that under strong light stress (700 PAR), the thylakoid morphology is abnormal in the leaves, the antioxidant system is damaged and the superoxide dismutase (SOD) and catalase (CAT) contents are lower than those of leaves under the 300 PAR light treatment, the synthesis of photosystem I (PSI) and photosystem II (PSII) protein members and the stability of the photosystem complex are reduced, and starch accumulation is also reduced. However, strong light stress promotes the endogenous synthesis of abscisic acid (ABA) and soluble sugar accumulation. Our research revealed that 300 PAR is a relatively appropriate growth light condition and that strong light stress (700 PAR) causes damage to the photosystem of C. petelotii. This is the first study to determine the molecular mechanism of the C. petelotii leaf responds to strong light stress at the transcription level and provides important insights for the future scientific cultivation of C. petelotii.
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This study was supported by the Fundamental Research Funds for Guangxi Forestry Research Institute (201827).
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11756_2022_1172_MOESM1_ESM.jpg
Supplementary file1 (JPG 283 KB) Supplement Fig. S1 Contents of carotenoids, chlorophyll a, and chlorophyll b in the leaves of Camellia petelotii (Merr.) Sealy under the treatment of E (700 PAR), M (300 PAR), and L (100 PAR) for 0, 5, and 10 hours. All bars represent the means ± s.d., and those labeled with a letter are significantly different at P < 0.05 by Duncan’s test. Three biological replicates in the experiment were performed. For each biological replication, five leaves of similar height were selected from the same Camellia petelotii (Merr.) Sealy plant
11756_2022_1172_MOESM2_ESM.jpg
Supplementary file2 (JPG 327 KB) Supplement Fig. S2 Contents of IAA, MeJA, Zeatin, MeSA, and GA3 phytohormones in the leaves of Camellia petelotii (Merr.) Sealy under the treatment of E (700 PAR), M (300 PAR), and L (100 PAR) for 0, 5, and 10 hours. All bars represent the means ± s.d., and those labeled with a letter are significantly different at P < 0.05 by Duncan’s test. Three biological replicates in the experiment were performed. For each biological replication, five leaves of similar height were selected from the same Camellia petelotii (Merr.) Sealy plant
11756_2022_1172_MOESM3_ESM.jpg
Supplementary file3 (JPG 87 KB) Supplement Fig. S3 Contents of MDA (The malondialdehyde) in the leaves of Camellia petelotii (Merr.) Sealy under the treatment of E (700 PAR), M (300 PAR), and L (100 PAR) for 0, 5, and 10 hours. All bars represent the means ± s.d., and those labeled with a letter are significantly different at P < 0.05 by Duncan’s test. Three biological replicates in the experiment were performed. For each biological replication, five leaves of similar height were selected from the same Camellia petelotii (Merr.) Sealy plant
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Huang, X., Qin, B., Xia, S. et al. A comparative study on the effects of strong light stress on the photosynthetic characteristics of the shade plant Camellia petelotii (Merr.) Sealy. Biologia 77, 3455–3468 (2022). https://doi.org/10.1007/s11756-022-01172-x
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DOI: https://doi.org/10.1007/s11756-022-01172-x