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Kinetics of Denaturation and Effects of Surfactants and Polyethylene Glycol on Soybean Esterase (Glycine max L) Stability

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Journal of the American Oil Chemists' Society

Abstract

The objective of this work was to evaluate the kinetics and thermodynamics parameters and the effects of anionic, cationic and nonionic surfactants and polyethylene glycol on the activity and stability of a crude esterase extracted from soybeans (Glycine max L.). The activation energy for thermal inactivation was calculated from the Arrhenius plot was found to be 59.4 kJ mol−1 and the ΔH* 56.82 kJ mol−1 at 40 °C, which was the optimum temperature for enzyme activity. The ΔS* and ΔG* of the enzyme were found to be 61.67 kJ mol−1 and 15.50 J mol−1 K−1, respectively, at the optimum temperature. The activity was only enhanced by the cationic surfactants cetyltrimethylammonium bromide and tetradecylmethylammonium bromide at a concentration of 3.0 mM. The anionic surfactant showed a positive effect on enzyme activity at the concentrations of 1.5 and 3.0 mM. Aqueous PEG (polyethylene glycols) solutions activated the esterase, and maximum activation (170 %) occurred with the addition of 6 kDa PEG. PEG with molecular weights of 0.4 and 10 kDa enhanced enzyme stability at 40 °C.

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Authors and Affiliations

  1. Biochemistry and Biotechnology Department, State University of Londrina (UEL), P.O. 1011, Londrina, PR, 86.057-970, Brazil

    Márcio de Barros & Maria Antonia P. C. Celligoi

  2. Food Science Department, School of Food Engineering, University of Campinas (UNICAMP), P.O. Box: 6121, Campinas, SP, 3083-862, Brazil

    Gabriela Alves Macedo

Authors
  1. Márcio de Barros
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  2. Maria Antonia P. C. Celligoi
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  3. Gabriela Alves Macedo
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Correspondence to Márcio de Barros.

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de Barros, M., Celligoi, M.A.P.C. & Macedo, G.A. Kinetics of Denaturation and Effects of Surfactants and Polyethylene Glycol on Soybean Esterase (Glycine max L) Stability. J Am Oil Chem Soc 93, 37–44 (2016). https://doi.org/10.1007/s11746-015-2755-8

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  • DOI: https://doi.org/10.1007/s11746-015-2755-8

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