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Dissimilar Properties of Vaccenic Versus Elaidic Acid in β-Oxidation Activities and Gene Regulation in Rat Liver Cells

  • Original Article
  • Published:
Lipids

Abstract

Vaccenic acid (trans-11-C18:1) chemically resembles elaidic acid (trans-9-C18:1) which is assumed to increase the risk of cardiovascular diseases, and thus could exert similar effects. Possible different oxidation rates of vaccenic versus elaidic acid were checked in muscles and liver, and through related gene expression in normal rat liver cells. In hepatic mitochondria, carnitine palmitoyltransferase (CPT) I exhibited comparable activity rates with both trans-isomers. CPT II activity was 30% greater (P < 0.05) with vaccenic than with elaidic acid as nonesterified fatty acids (NEFAs) or acyl-CoAs. Activity of the first β-oxidation step was similar between the isomers in all the tissue slices and liver extracts assayed. Respiration rates were comparable with both trans-isomers as NEFAs in various liver extracts, but were 30% greater (P < 0.05) with vaccenoyl-CoA than with elaidoyl-CoA in liver mitochondria. Vaccenic acid was oxidised 25% more (P < 0.05) by liver peroxisomes than elaidic acid. In hepatocytes cultured with trans- and corresponding cis-C18:1 isomers, gene expression of CPT I, hydroxyacyl-CoA dehydrogenase and hydroxymethylglutaryl-CoA synthase was at least 100% increased (P < 0.05), but was unchanged with vaccenic acid, relative to controls. In conclusion, the position and geometry of the double bonds in acyl chains are suggested to confer on vaccenic and elaidic acid specific biochemical properties that might differently affect their fates in tissues.

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Abbreviations

ACO:

Acyl-CoA oxidase

ACS:

Acyl-CoA synthetase

BSA:

Bovine serum albumin

CPT:

Carnitine palmitoyltransferase

FA:

Fatty acid

HAD:

Hydroxyacyl-CoA dehydrogenase

HMG:

Hydroxymethylglutaryl (in HMG-CoA synthase)

NEFA:

Nonesterified FA

PFAOS:

Peroxisomal FA-oxidising system

PPAR:

Peroxisome proliferator-activated receptor

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Acknowledgments

We thank Mr Jean-Michel Chardigny DR INRA, Mr Koenraad Duhem, Mrs Corinne Marmonier for helpful discussions, and Mrs Monique Baudoin for figure construction and typing of the manuscript. This work was supported by grants from the CNIEL (Paris) and the Region Bourgogne (Dijon), France.

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Authors and Affiliations

  1. Faculté des Sciences Gabriel, UMR 866, INSERM-UB, Equipe Physiopathologie des dyslipidémies, Université de Bourgogne, 21000, Dijon, France

    Zhen-Yu Du, Pascal Degrace, Joseph Gresti & Pierre Clouet

  2. National Institute of Nutrition and Seafood Research, 5817, Bergen, Norway

    Zhen-Yu Du

  3. CEA, iBiTecS, Service Chimie Bioorganique et Marquage, 91191, Gif sur Yvette, France

    Olivier Loreau

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  1. Zhen-Yu Du
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  2. Pascal Degrace
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  3. Joseph Gresti
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  4. Olivier Loreau
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  5. Pierre Clouet
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Correspondence to Pierre Clouet.

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Du, ZY., Degrace, P., Gresti, J. et al. Dissimilar Properties of Vaccenic Versus Elaidic Acid in β-Oxidation Activities and Gene Regulation in Rat Liver Cells. Lipids 45, 581–591 (2010). https://doi.org/10.1007/s11745-010-3428-x

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  • DOI: https://doi.org/10.1007/s11745-010-3428-x

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