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Metabolism of trans fatty acids by hepatocytes

  • Published:
Lipids

Abstract

The present work was undertaken to study the metabolism of fatty acids with trans double bonds by rat hepatocytes. In liver mitochondria, elaidoyl-CoA was a poorer substrate for carnitine palmitoyltransferase I (CPT-I) than oleoyl-CoA. Likewise, incubation, of hepatocytes with oleic acid produced a more pronounced stimulation of CPT-I than incubation with trans fatty acids. This was not due to a differential effect of cis and trans fatty acids on acetyl-CoA carboxylase (ACC) activity and malonyl-CoA levels. Elaidic acid was metabolized by hepatocytes at a higher rate than oleic acid. Surprisingly, compared to oleic acid, elaidic acid was a better substrate for mitochondrial and, especially, peroxisomal oxidation, but a poorer substrate for cellular and very low density lipoprotein triacylglycerol synthesis. Results thus show that trans fatty acids are preferentially oxidized by hepatic peroxisomes, and that the ACC/malonyl-CoA/CPT-I system for coordinate control of fatty acid metabolism is not responsible for the distinct hepatic utilization of cis and trans fatty acids.

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Abbreviations

ACC:

acetyl-CoA carboxylase

CPT-I:

carnitine palmitoyl-transferase I

TDGA:

tetradecylglycidic acid

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, 28040, Madrid, Spain

    Manuel Guzmán & Teresa Gómez del Pulgar

  2. Laboratory of Veterinary Biochemistry, Ultrecht University, P.O. Box 80.176, 3508, TD Utrecht, The Netherlands

    Wil Klein & Math J. H. Geelen

Authors
  1. Manuel Guzmán
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  2. Wil Klein
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  3. Teresa Gómez del Pulgar
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  4. Math J. H. Geelen
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Guzmán, M., Klein, W., Gómez del Pulgar, T. et al. Metabolism of trans fatty acids by hepatocytes. Lipids 34, 381–386 (1999). https://doi.org/10.1007/s11745-999-0376-6

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  • DOI: https://doi.org/10.1007/s11745-999-0376-6

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