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Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL-Induced Mouse RAW264.7 Macrophage-Derived Foam Cells

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Lipids

Abstract

Synthetic activators of peroxisome proliferator-activated receptors (PPAR)-α and -γ are capable of reducing macrophage foam cell cholesterol accumulation through the activation of genes involved in cholesterol homeostasis. Since conjugated linoleic acids (CLA) were also demonstrated to activate PPARα and PPARγ in vivo and in vitro, we tested the hypothesis that CLA are also capable of reducing macrophage foam cell cholesterol accumulation. Thus, mouse RAW264.7 macrophage-derived foam cells were treated with CLA isomers, c9t11-CLA and t10c12-CLA, and linoleic acid (LA), as reference fatty acid, and analyzed for the concentrations of free and esterified cholesterol, cholesterol efflux and expression of genes involved in cholesterol homeostasis (CD36, ABCA1, LXRα, NPC-1, and NPC-2). Treatment with c9t11-CLA and t10c12-CLA, but not LA, lowered cholesterol accumulation, stimulated acceptor-dependent cholesterol efflux, and increased relative mRNA concentrations of CD36, ABCA1, LXRα, NPC-1, and NPC-2 (P < 0.05). In conclusion, the present study showed that CLA isomers reduce cholesterol accumulation in RAW264.7 macrophage-derived foam cells presumably by enhancing lipid acceptor-dependent cholesterol efflux.

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Abbreviations

ABCA1:

ATP-binding cassette transporter A1

AcLDL:

Acetylated LDL

CLA:

Conjugated linoleic acid

HDL:

High-density lipoprotein

LA:

Linoleic acid

LDL:

Low-density lipoprotein

LXRα:

Liver X receptor α

NPC:

Niemann Pick type C

PPAR:

Peroxisome proliferator-activated receptor

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Authors and Affiliations

  1. Institute of Agricultural and Nutritional Sciences, Martin-Luther-University of Halle-Wittenberg, Emil-Abderhalden-Strasse 26, 06108, Halle (Saale), Germany

    Robert Ringseis, Gaiping Wen, Daniela Saal & Klaus Eder

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  1. Robert Ringseis
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  2. Gaiping Wen
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  3. Daniela Saal
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  4. Klaus Eder
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Correspondence to Robert Ringseis.

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Ringseis, R., Wen, G., Saal, D. et al. Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL-Induced Mouse RAW264.7 Macrophage-Derived Foam Cells. Lipids 43, 913–923 (2008). https://doi.org/10.1007/s11745-008-3226-x

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  • DOI: https://doi.org/10.1007/s11745-008-3226-x

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