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Conjugated Linoleic Acids Can Change Phagocytosis of Human Monocytes/Macrophages by Reduction in Cox-2 Expression

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Lipids

Abstract

Prostaglandin E2 produced endogenously (by cyclooxygenases) can regulate macrophage phagocytosis. Cyclooxygenase activity reduction (mainly through inhibition of inducible Cox-2) can induce PGE2 synthesis depression and can activate the phagocytosis process. There are no reports in the literature explaining whether conjugated linoleic acid dienes (trans-10, cis-12 CLA and cis-9, trans-11 CLA) modify the phagocytic activity of human macrophages. For the purpose of this study, monocytes were isolated from venous blood, incubated for 7 days with 30 μM CLAs, and then (in some experiments) LPS (1 μg/mL) was added to the medium. Subsequently, monocyte/macrophage phagocytosis, NF-κB transcription factor activity, Cox-2 and PPARγ mRNA expression (and the amounts of Cox-2 and PPARγ proteins) and PGE2 synthesis were determined. Both CLA isomers increased macrophage phagocytosis through inhibition of Cox-2 expression (might by inactivation the NF-κB pathway). The inhibition of mRNA Cox-2 expression contributed (particularly with respect to trans-10, cis-12 CLA) to a decrease in protein Cox-2 synthesis and to reduction of prostaglandin E2 content in the cell. The inhibition of PGE2 synthesis (by CLA treatment) enhanced the phagocytosis process in macrophages.

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Abbreviations

CLA:

Conjugated linoleic acid

Cox:

Cyclooxygenase

LPS:

Lipopolysaccharide

NF-κB:

Nuclear factor κB

PPAR:

Peroxysome proliferator activated receptors

PGE2 :

Prostaglandin E2

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Acknowledgments

Supported by grant no. 3PO5B 11723 from the State Committee for Scientific Research, Poland. M. Baśkiewicz-Masiuk is a scholarship holder of the Foundation for Polish Science.

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Authors and Affiliations

  1. Department of Biochemistry, Pomeranian Medical University, Szczecin, Poland

    Ewa Stachowska, Violetta Dziedziejko & Dariusz Chlubek

  2. Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

    Magdalena Baśkiewicz-Masiuk & Bogusław Machaliński

  3. Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Szczecin, Poland

    Grażyna Adler

  4. Department of Medical Chemistry, Pomeranian Medical University, Szczecin, Poland

    Joanna Bober

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  1. Ewa Stachowska
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  2. Magdalena Baśkiewicz-Masiuk
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Correspondence to Ewa Stachowska.

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Stachowska, E., Baśkiewicz-Masiuk, M., Dziedziejko, V. et al. Conjugated Linoleic Acids Can Change Phagocytosis of Human Monocytes/Macrophages by Reduction in Cox-2 Expression. Lipids 42, 707–716 (2007). https://doi.org/10.1007/s11745-007-3072-2

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  • DOI: https://doi.org/10.1007/s11745-007-3072-2

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