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Lymphatic transport in rats of interesterified oils containing conjugated linoleic acids

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Lipids

Abstract

In this study we examined the lymphatic transport in rats of FA after administration of interesterified oils containing CLA, with emphasis on the location of CLA and octanoic acid in the TAG. The oils were produced by enzymatic interesterification. Eight oils with different structures or FA profiles were examined in this study: MCM, CMC, OCO, and COC, where M was expected to be octanoic acid and O oleic acid. In group 1, C was CLA as a mixture of the two CLA isomers c9, t11 or t10,c12, and in group 2, C was mainly the isomer t10,c12. Rats were subjected to cannulation of the mesenteric lymph duct, and the following day they were intragastrically administered one of the oils and lymph samples were collected for 24 h. The lymphatic transport of total FA from 0 to 8 h in group 1 was significantly (P<0.05) higher for the OCO-1 and the COC-1 oils than for the CMC-1 oil. Similarly, in group 2 the transport was higher for the OCO-2 oil than for the CMC-2 oil. The recovery of both of the CLA isomers examined was similar (50–70%) and independent of the isomer, oil structure, and FA profile, whereas more octanoic acid was recovered from the CMC oils than from the MCM oils. The results indicated that the FA profiles and the position of octanoic acid had only a minor influence on the absorption of CLA.

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Abbreviations

CMC:

structured TAG with CLA primarily in the sn-1/3-positions and 8∶0 in the sn-2-position

COC:

structured TAG with the major proportion of CLA in the sn-1/3-positions and 18∶1n−9 in the sn-2-position

ECN:

equivalent carbon number

IAUC:

incremental area under the curve

LCFA:

long-chain fatty acids

LLL:

TAG with three LCFA

LML:

structured TAG with LCFA in the sn-1/3-positions and MCFA in the sn-2-position

MCFA:

medium-chain fatty acids

MCM:

structured TAG with the major proportion of CLA in the sn-2-position and 8∶0 in the sn-1/3-positions

MLM:

structured TAG with MCFA in the sn-1/3-positions and LCFA in the sn-2-position

OCO:

structured TAG with CLA primarily in the sn-2-position and 18∶1n−9 in the sn-1/3-positions

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Authors and Affiliations

  1. Biochemistry and Nutrition Group, BioCentrum-DTU, Technical University of Denmark, Bldg. 224, DK-2800, Lyngby, Denmark

    Ellen Marie Straarup, Trine Porsgaard, Huiling Mu, Christina H. Hansen & Carl-Erik Høy

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  1. Ellen Marie Straarup
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  2. Trine Porsgaard
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  3. Huiling Mu
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  4. Christina H. Hansen
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  5. Carl-Erik Høy
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Correspondence to Trine Porsgaard.

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Carl-Erik Høy, of Lyngby, Denmark, died on February 8, 2003, just before his 56th birthday.

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Straarup, E.M., Porsgaard, T., Mu, H. et al. Lymphatic transport in rats of interesterified oils containing conjugated linoleic acids. Lipids 40, 677–684 (2005). https://doi.org/10.1007/s11745-005-1430-0

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