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Clinical trial results support a preference for using CLA preparations enriched with two isomers rather than four isomers in human studies

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Lipids

Abstract

CLA mixtures are now commercially available. They differ from each other with respect to their content of CLA isomers and their degree of purification. As a group of natural FA, CLA have been widely assumed to be safe. However, the suspected presence of both impurities and particular isomers might induce undesirable side effects. Despite this potential health risk, only a few CLA preparations have been tested under rigorous conditions for clinical efficacy and safety. Based on the limited results available, it is possible to suggest that preparations enriched in c9,t11 and t10,c12 isomers are preferable for human consumption compared to preparations containing four isomers, in terms both of safety and efficacy.

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Abbreviations

c :

cis configuration

LA:

linoleic acid

t :

trans configuration

References

  1. Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1987) Anticarcinogens from Fried Ground Beef: Heat-Altered Derivatives of Linoleic Acid, Carcinogenesis 8, 1881–1887.

    PubMed  CAS  Google Scholar 

  2. Ip, C., Ip, M.M., Loftus, T., Shoemaker, S., and Shea-Eaton, W. (2000) Induction of Apoptosis by Conjugated Linoleic Acid in Cultured Mammary Tumor Cells and Premalignant Lesions of the Rat Mammary Gland, Cancer Epidemiol. Biomarkers Prev. 9, 689–696.

    PubMed  CAS  Google Scholar 

  3. Kritchevsky, D. (2000) Antimutagenic and Some Other Effects of Conjugated Linoleic Acid, Br. J. Nutr. 83, 459–465.

    PubMed  CAS  Google Scholar 

  4. Pariza, M.W., Park, Y., and Cook, M.E. (2000) Mechanisms of Action of Conjugated Linoleic Acid: Evidence and Speculation, Proc. Soc. Exp. Biol. Med. 223, 8–13.

    Article  PubMed  CAS  Google Scholar 

  5. Lee, K.N., Kritchevsky, D., and Pariza, M.W. (1994) Conjugated Linoleic Acid and Atherosclerosis in Rabbits, Atherosclerosis 108, 19–25.

    Article  PubMed  CAS  Google Scholar 

  6. Nicolosi, R.J., Rogers, E.J., Kritchevsky, D., Scimeca, J.A., and Huth, P.J. (1997) Dietary Conjugated Linoleic Acid Reduces Plasma Lipoproteins and Early Aortic Atherosclerosis in Hypercholesterolemic Hamsters, Artery 22, 266–277.

    PubMed  CAS  Google Scholar 

  7. Cook, M.E., Miller, C.C., Park, Y., and Pariza, M. (1993) Immune Modulation by Altered Nutrient Metabolism: Nutritional Control of Immune-Induced Growth Depression, Poult. Sci. 72, 1301–1305.

    PubMed  CAS  Google Scholar 

  8. Miller, C.C., Park, Y., Pariza, M.W., and Cook, M.E. (1994) Feeding Conjugated Linoleic Acid, Biochem. Biophys. Res. Commun. 198, 1107–1112.

    Article  PubMed  CAS  Google Scholar 

  9. Chin, S.F., Storkson, J.M., Liu, W., Albright, K.J., and Pariza, M.W. (1994) Conjugated Linoleic Acid (9,11- and 10,12-octadecadienoic acid) Is Produced in Conventional but Not Germ-Free Rats Fed Linoleic Acid, J. Nutr. 124, 694–701.

    PubMed  CAS  Google Scholar 

  10. DeLany, J.P., Blohm, F., Truett, A.A., Scimeca, J.A., and West, D.B. (1999) Conjugated Linoleic Acid Rapidly Reduces Body Fat Content in Mice Without Affecting Energy Intake, Am. J. Physiol. 276, R1172-R1179.

    PubMed  CAS  Google Scholar 

  11. Park, Y., Storkson, J.M., Albright, K.J., Liu, W., and Pariza, M.W. (1999) Evidence That the trans-10,cis-12 Isomer of Conjugated Linoleic Acid Induces Body Composition Changes in Mice, Lipids 34, 235–241.

    Article  PubMed  CAS  Google Scholar 

  12. West, D.B., DeLany, J.P., Camet, P.M., Blohm, F., Truett, A.A. and Scimeca, J. (1998) Effects of Conjugated Linoleic Acid on Body Fat and Energy Metabolism in the Mouse, Am. J. Physiol. 275, R667-R672.

    PubMed  CAS  Google Scholar 

  13. Szymczyk, B., Pisulewski, P.M., Szczurek, W., and Hanczakowski, P. (2001) Effects of Conjugated Linoleic Acid on Growth Performance, Feed Conversion Efficiency, and Subsequent Carcass Quality in Broiler Chickens, Br. J. Nutr. 85, 465–473.

    PubMed  CAS  Google Scholar 

  14. Gavino, V.C., Gavino, G., Leblanc, M.J., and Tuchweber, B., (2000) An Isomeric Mixture of Conjugated Linoleic Acids but Not Pure cis-9,trans-11-Octadecadienoic Acid Affects Body Weight Gain and Plasma Lipids in Hamsters, J. Nutr. 130, 27–29.

    PubMed  CAS  Google Scholar 

  15. Dugan, M.E.R., Aalhus, J.L., Schaefer, A.L., and Kramer, J.K.G. (1997) The Effects of Conjugated Linoleic Acid on Fat to Lean Repartitioning and Feed Conversion in Pigs, Can. J. Anim. Sci. 77, 723–725.

    Article  CAS  Google Scholar 

  16. Sehat, N., Yurawecz, M.P., Roach, J.A., Mossoba, M.M., Kramer, J.K., and Ku, Y. (1998) Silver Ion High-Performance Liquid Chromatographic Separation and Identification of Conjugated Linoleic and Isomers, Lipids 33, 217–221.

    Article  PubMed  CAS  Google Scholar 

  17. Chin, S.F., Liu, W., Storkson, J.M., Ha, Y.L., and Pariza, M.W. (1992) Dietary Sources of Conjugated Linoleic Acid, a Newly Recognized Class of Anticarcinogens, J. Food Comp. Anal. 5, 185–197.

    Article  CAS  Google Scholar 

  18. McGuire, M.K., McGuire, M.A., Ritzenthaler, K., and Schultz, T.D. (1999) Dietary Sources and Intakes of Conjugated Linoleic Acid in Humans, in Advances in Conjugated Linoleic Acid Research (Yurawecz, M.P., Mossoba, M.M., Kramer, J.K.G., Pariza, M.W., and Nelson, G.J., eds.), Vol. 1, pp. 369–377, AOCS Press, Champaign.

    Google Scholar 

  19. Yurawecz, M.P., Sehat, N., Mossoba, M.M., Roach, J.A.G. Kramer, J.K.G., and Ku, Y. (1999) Variations in Isomer Distribution in Commercially Conjugated Linoleic Acid, Fett/Lipid 101, 277–282.

    Article  CAS  Google Scholar 

  20. Padley, F.B., Gunstone, F.D., and Harwood, J.L. (1994) Occurrence and Characteristics of Oils and Fats, in The Lipid Handbook (Gunstone, F.D., Harwood, J.L., and Padley, F.B., eds.), pp. 47–224, Chapman & Hall, London.

    Google Scholar 

  21. Saebo, A. (2001) Commercial Production of Conjugated Linoleic Acid (CLA), Lipid Technol. Newsl., 9–13.

  22. Baumgard, L.H., Corl, B.A., Dwyer, D.A., Saebo, A., and Bauman, D.E. (2000) Identification of the Conjugated Linoleic Acid Isomer That Inhibits Milk Fat Synthesis, Am. J. Physiol Regul. Integr. Comp Physiol 278, R179-R184.

    PubMed  CAS  Google Scholar 

  23. Christie, W.W., Dobson, G., and Gunstone, F.D. (1997) Isomers in Commerical Samples of Conjugated Linoleic Acid, Lipids 32, 1231.

    Article  PubMed  CAS  Google Scholar 

  24. Sébédio, J.-L., Juaneda, P., Dobson, G., Ramilison, I., Martin, J.C., Chardigny, J.M., and Christie, W.W. (1997) Metabolites of Conjugated Isomers of Linoleic Acid (CLA) in the Rat, Biochim. Biophys. Acta 1345, 5–10.

    PubMed  Google Scholar 

  25. Pariza, M.W., Park, Y., and Cook, M.E. (2001) The Biologically Active Isomers of Conjugated Linoleic Acid, Prog. Lipid Res. 40, 283–298.

    Article  PubMed  CAS  Google Scholar 

  26. Riserus, U., Berglund, L., and Vessby, B. (2001) Conjugated Linoleic Acid (CLA) Reduced Abdominal Adipose Tissue in Obese Middle-Aged Men with Signs of the Metabolic Syndrome: A Randomized Controlled Trial, Int. J. Obes. Relat. Metab. Disord. 25, 1129–1135.

    Article  PubMed  CAS  Google Scholar 

  27. Banni, S., Angioni, E., Casu, V., Melis, M.P., Carta, G., Corongiu, F.P., Thompson, H., and Ip, C. (1999) Decrease in Linoleic Acid Metabolites as a Potential Mechanism in Cancer Risk Reduction by Conjugated Linoleic Acid, Carcinogenesis 20, 1019–1024.

    Article  PubMed  CAS  Google Scholar 

  28. Ip, C., Chin, S.F., Scimeca, J.A., and Pariza, M.W. (1991) Mammary Cancer Prevention by Conjugated Dienoic Derivative of Linoleic Acid, Cancer Res. 51, 6118–6124.

    PubMed  CAS  Google Scholar 

  29. Noone, E.J., Roche, H.M., Nugent, A.P., and Gibney, M.J. (2002) The Effect of Dietary Supplementation Using Isomeric Blends of Conjugated Linoleic Acid on Lipid Metabolism in Healthy Human Subjects, Br. J. Nutr. 88, 243–251.

    Article  PubMed  CAS  Google Scholar 

  30. Kramer, J.K.G., Sehat, N., Dugan, M.E., Mossoba, M.M., Yurawecz, M.P., Roach, J.A., Eulitz, K., Aalhus, J.L., Schaefer, A.L., and Ku, Y. (1998) Distributions of Conjugated Linoleic Acid (CLA) Isomers in Tissue Lipid Classes of Pigs Fed a Commercial CLA Mixture Determined by Gas Chromatography and Silver Ion-High-Performance Liquid Chromatography, Lipids 33, 549–558.

    Article  PubMed  CAS  Google Scholar 

  31. Parodi, P.W. (1994) Conjugated Linoleic Acid: An Anticarcinogenic Fatty Acid Present in Milk Fat, Aust. J. Dairy Technol. 49, 93–97.

    CAS  Google Scholar 

  32. Ip, C., Scimeca, J.A., and Thompson, H.J. (1994) Conjugated Linoleic Acid. A Powerful Anticarcinogen from Animal Fat Sources, Cancer 74, 1050–1054.

    Article  PubMed  CAS  Google Scholar 

  33. Berven, G., Bye, A., Hals, O., Blankson, H., Faggertun, H., Thom, E., Wadstein, J., and Gudmundsen, O. (2000) Safety of Conjugated Linoleic Acid (CLA) in Overweight or Obese Human Volunteers, Eur. J. Lipid Sci. Technol. 102, 455–462.

    Article  CAS  Google Scholar 

  34. Blankson, H., Stakkestad, J.A., Fagertun, H., Thom, E., Wadstein, J., and Gudmundsen, O. (2000) Conjugated Linoleic Acid Reduces Body Fat Mass in Overweight and Obese Humans, J. Nutr. 130, 2943–2948.

    PubMed  CAS  Google Scholar 

  35. Benito, P., Nelson, G.J., Kelley, D.S., Bartolini, G., Schmidt, P.C., and Simon, V. (2001) The Effect of Conjugated Linoleic Acid on Platelet Function, Platelet Fatty Acid Composition, and Blood Coagulation in Humans, Lipids 36, 221–227.

    Article  PubMed  CAS  Google Scholar 

  36. Benito, P., Nelson, G.J., Kelley, D.S., Bartolini, G., Schmidt, P.C., and Simon, V. (2001) The Effect of Conjugated Linoleic Acid on Plasma Lipoproteins and Tissue Fatty Acid Composition in Humans, Lipids 36, 229–236.

    Article  PubMed  CAS  Google Scholar 

  37. Medina, E.A., Horn, W.F., Keim, N.L., Havel, P.J., Benito, P., Kelley, D.S., Nelson, G.J., and Erickson, K.L. (2000) Conjugated Linoleic Acid Supplementation in Humans: Effects on Circulating Leptin Concentrations and Appetite, Lipids 35, 783–788

    PubMed  CAS  Google Scholar 

  38. Thom, E., Wadstein, J., and Gudmundsen, O. (2001) Conjugated Linoleic Acid Reduces Body Fat in Healthy Exercising Humans, J. Int. Med. Res. 29, 392–396.

    PubMed  CAS  Google Scholar 

  39. Zambell, K.L., Keim, N.L., Van Loan, M.D., Gale, B., Benito, P., Kelley, D.S., and Nelson, G.J. (2000) Conjugated Linoleic Acid Supplementation in Humans: Effects on Body Composition and Energy Expenditure, Lipids 35, 777–782.

    PubMed  CAS  Google Scholar 

  40. Zambell, K.L., Horn, W.F., and Keim, N.L. (2001) Conjugated Linoleic Acid Supplementation in Humans: Effects on fatty Acid and Glycerol Kinetics, Lipids 36, 767–772.

    Article  PubMed  CAS  Google Scholar 

  41. Jahreis, G., and Loeffelholz, C.V. (2000) Influence of CLA Supplementation on Body Composition and Strength, in CLA: What's Going On? (Centre d'Etude sur la Recherche et l'Innovation, ed.), Vol. 4, pp. 2–3, CERIN, Paris.

    Google Scholar 

  42. Smedman, A., and Vessby, B. (2001) Conjugated Linoleic Acid Supplementation in Humans—Metabolic Effects, Lipids 36, 773–781.

    Article  PubMed  CAS  Google Scholar 

  43. Lowery, L.M., Appicelli, P., and Lemon, P.W.R. (1998) Conjugated Linoleic Acid Enhances Muscle Size, and Strength Gains in Novice Bodybuilders, Med. Sci. Sports Exerc. 30, 182 (abstract 1038).

    Article  Google Scholar 

  44. Mohede, I., Albers R., van der Wielen, R., Brink, L., and Dorovska-Taran, V. (2001) Immunomodulation: CLA Stimulates Antigen Specific Antibody Production in Humans, 1st International Conference on Conjugated Linoleic Acid (CLA), Ålesund, Norway, p. 12 (abstract).

  45. Mougios, V., Matsakas, A., Rings, S., Petridou, A., Sagredos, A., Melissopoulou, A., and Tsigilis, N. (2001) Effects of Supplementation with Conjugated Linoleic Acid on Human Body Fat and Serum Lipids, J. Nutr. Biochem. 12, 585–594.

    Article  PubMed  CAS  Google Scholar 

  46. Basu, S., Riserus, U., Turpeinen, A., and Vessby, B. (2000) Conjugated Linoleic Acid Induces Lipid Peroxidation in Men with Abnominal Obesity, Clin. Sci. (London) 99, 511–516.

    CAS  Google Scholar 

  47. Basu, S., Smedman, A., and Vessby, B. (2001) Isomer Specific Effects of Conjugated Linoleic Acid (CLA) on Lipid Peroxidation and Its Regulation by COX2 Inhibitor and Vitamin E in Humans, Free Radic. Biol. Med. 31 (Suppl. 1), 333 (abstract)

    Google Scholar 

  48. Basu, S., Smedman, A., and Vessby, B. (2000) Conjugated Linoleic Acid Induces Lipid Peroxidation in Humans, FEBS Lett. 468, 33–36.

    Article  PubMed  CAS  Google Scholar 

  49. Atkinson, R.L. (1999) Conjugated Linoleic Acid for Altering Body Composition and Treating Obesity, in Advances in Conjugated Linoleic Acid Research (Yurawecz, M.P., Mossoba, M.M., Kramer, J.K.G., Pariza, M.W., and Nelson, G.J., eds.), Vol. 1, pp. 348–353, AOCS Press, Champaign.

    Google Scholar 

  50. Riserus, U., Arner, P., Brismar, K., and Vessby, B. (2002) Treatment with Dietary trans10,cis12 Conjugated Linoleic Acid Causes Isomer-Specific Insulin Resistance in Obese Men with the Metabolic Syndrome, Diabetes Care 25, 1516–1521.

    PubMed  CAS  Google Scholar 

  51. Choi, Y., Kim, Y.C., Han, Y.B., Park, Y., Pariza, M.W., and Ntambi, J.M. (2000) The trans-10,cis-12 Isomer of Conjugated Linoleic Acid Down Regulates Stearoyl-CoA Desaturase 1 Gene Expression 3T3-L1 Adipocytes, J. Nutr. 130, 1920–1924.

    PubMed  CAS  Google Scholar 

  52. Ryder, J.W., Portocarrero, C.P., Song, X.M., Cui, L., Yu, M., Combatsiaris, T., Galuska, D., Bauman, D.E., Barbano, D.M., Charron, M.J., et al. (2001) Isomer-Specific Antidiabetic Properties of Conjugated Linoleic Acid. Improved Glucose Tolerance, Skeletal Muscle Insulin Action, and UCP-2 Gene Expression, Diabetes 50, 1149–1157.

    PubMed  CAS  Google Scholar 

  53. Belury, M.A. (2002) Dietary Conjugated Linoleic Acid in Health: Physiological Effects and Mechanisms of Action, Annu. Rev. Nutr. 22, 505–531.

    Article  PubMed  CAS  Google Scholar 

  54. Kelley, D.S., Taylor, P.C., Rudolph, I.L., Benito, P., Nelson, G.J., Mackey, B.E., and Erickson, K.L. (2000) Dietary Conjugated Linoleic Acid Did Not Alter Immune Status in Young Healthy Women, Lipids 35, 1065–1071.

    Article  PubMed  CAS  Google Scholar 

  55. Kelley, D.S., Simon, V.A., Taylor, P.C., Rudolph, I.L., Benito, P., Nelson, G.J., Mackey, B.E., and Erickson, K.L. (2001) Dietary Supplementation with Conjugated Linoleic Acid Increased Its Concentration in Human Peripheral Blood Mononuclear Cells, but Did Not Alter Their Function, Lipids 36, 669–674.

    Article  PubMed  CAS  Google Scholar 

  56. Kreider, R.B., Ferreira, M.P., Greenwood, M., Wilson, M., and Almada, A.L. (2002) Effects of Conjugated Linoleic Acid Supplementation During Resistance-Training on Body Composition, Bone Density, Strength, and Selected Hematological Markers, J. Strength Cond. Res. 3, 325–334.

    Article  Google Scholar 

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  1. Scandinavian Clinical Research AS, Gåsevikveien 8, P.O. Box 135, 2027, Kjeller, Norway

    Jean-Michel Gaullier, Grethe Berven, Henrietta Blankson & Ola Gudmundsen

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  1. Jean-Michel Gaullier
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  2. Grethe Berven
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  3. Henrietta Blankson
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  4. Ola Gudmundsen
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Correspondence to Jean-Michel Gaullier.

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Gaullier, JM., Berven, G., Blankson, H. et al. Clinical trial results support a preference for using CLA preparations enriched with two isomers rather than four isomers in human studies. Lipids 37, 1019–1025 (2002). https://doi.org/10.1007/s11745-002-0995-y

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  • DOI: https://doi.org/10.1007/s11745-002-0995-y

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