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Flavonoid biosynthesis regulation for leaf coloring of Cyclocarya paliurus

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Abstract

Cyclocarya paliurus leaves contain abundant flavonoids with beneficial bioactivities and antioxidant activities. Red leaves of Cyclocarya paliurus have been considered to have higher flavonoids content, while the flavonoid derivatives accumulation pattern was still unclear. To uncover the flavonoids variation mechanism during red young leaf growing, transcriptome and metabolome analysis on Cyclocarya paliurus clone with red leaves was made. Leaves samples of young red leaf, half red leaf and old green leaf stage were researched. The result showed that young red leaf contained higher anthocyanin and quercetin contents, while lower kaempferol and catechins contents compared to old green leaf. And flavonoid biosynthesis-related unigenes (UDP-flavonoid glucosyl transferase) were identified for promoting anthocyanin accumulation in the flavonoid biosynthesis pathway. Besides, transcription factors were also found to influence the flavonoids variation pattern in the red leave of C. paliurus. These findings provide information about the relationship between flavonoids and leaf color, and will be useful for C. paliurus selection.

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Data availability

The datasets generated during and/or analyzed during the current study are not publicly available due the data also form part of an ongoing study but are available from the corresponding author on reasonable request.

Abbreviations

C. Paliurus :

Cyclocarya paliurus

LC1:

The C. paliurus clone seedlings with red young leaves were used as plant materials

FPKM:

The fragments per kilobase of exon per million fragments mapped

MYB TFs:

MYB transcription factors

A:

Old green leaf period

B:

Semi red leaf period

C:

Young red leaf period

ABLog2 FC:

Log2 fold change between A and B stage

BCLog2 FC:

Log2 fold change between B and C stage

ACLog2 FC:

Log2 fold change between A and C stage

PAL:

Phenylalanine ammonia-lyase

C4H:

Cinnamic acid 4-hydroxylase

4CL:

4 Coumarate CoA ligase

CHS:

Chalcone synthase

CHI:

Chalcone isomerase

F3H:

Flavanone 3-hydroxylase

CYP75A:

Flavonoid 3ʹ, 5ʹ-hydroxylase

CYP75B1:

Flavonoid 3ʹ-monooxygenase

FLS:

Flavonol synthase

UFGT:

UDP-flavonoid glucosyl transferase

DFR:

Dihydroflavonol 4-reductase

LAR:

Leucoanthocyanidin reductase

ANS:

Anthocyanidin synthase

ANR:

Anthocyanidin reductase

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Funding

This work was supported by the National Natural Science Foundation of China (32201541), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Doctorate Fellowship Foundation of Nanjing Forestry University. The funders had no role in studying design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. College of Forestry, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Caowen Sun, Shengzuo Fang & Xulan Shang

  2. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Caowen Sun, Shengzuo Fang & Xulan Shang

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  1. Caowen Sun
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  2. Shengzuo Fang
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Correspondence to Xulan Shang.

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Sun, C., Fang, S. & Shang, X. Flavonoid biosynthesis regulation for leaf coloring of Cyclocarya paliurus. Acta Physiol Plant 45, 91 (2023). https://doi.org/10.1007/s11738-023-03571-2

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