Abstract
Wild progenitors of grapevine constitute a diverse source of genetic variability which have been used as a potential rootstock to cope with different abiotic and biotic stresses. In Pakistan, wild grapevine accessions locally called Dakh, Fatati, Toran, Zarishk, and Kishmish naturally exist in mountainous and sub-mountainous regions of humid and sub-humid areas. However, many vineyards that are cultivated as own rooted crops have been affected by many environmental or edaphic conditions. To cope with these adverse conditions, it is the utmost need to cultivate highly productive Vitis varieties with suitable rootstocks, but compatibility is a major concern between these grafted plants. In the present study, in vitro cleft grafting was used to evaluate the compatibility of these wild grapevine accessions as rootstocks with other commercial scion varieties like King’s Ruby and Flame Seedless. Wild grapevine accessions Dakh, Kishmish, and Toran showed a relatively improved rate of compatibility with commercial scion varieties compared to Zarishk and Fatati accessions. Initially, a slower but stable process of graft compatibility between wild grapevine accessions and scion varieties was observed at 10 days after grafting. Differentiation of parenchymatous cells into vascular bundles started at 15 days after grafting, whereas complete formation of vascular bundles at graft junctions of wild grapevine accessions and commercial scion varieties was observed at 21 days after grafting. This initiation of vascular layers at the initial stage helped the improvement of shoot growth of scion varieties grafted on wild grapevine accessions Dakh and Kishmish. Moreover, a higher rate of root growth was observed in grafted wild grapevine accessions Dakh, Zarishk, and Kishmish compared to Fatati and Toran. Among both commercial scion varieties, King’s Ruby resulted (54–59%) as more effective when grafted with wild grapevine accessions.
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References
Assuncao M, Canas S, Cru S, Brazão J, Zanol GC, Eiras-Dias JE (2016) Graft compatibility of Vitis spp.: the role of phenolic acids and flavanols. Sci Hortic 207:140–145
Bantis F, Koukounaras A, Siomos AS, Dangitsis C (2020) Impact of scion and rootstock seedling quality selection on the vigor of watermelon-interspecific squash grafted seedlings. Agriculture 10(8):1–10
Bhogale S, Mahajan AS, Natarajan B, Rajabhoj M, Thulasiram HV, Banerjee AK (2014) MicroRNA156: a potential graft-transmissible microRNA that modulates plant architecture and tuberization in Solanum tuberosum ssp. andigena. Plant Physiol 164(2):1011–1027
Bidabadi SS, Afazel M, Sabbatini P (2017) Iranian grapevine rootstocks and hormonal effects on graft union, growth and antioxidant responses of asgari seedless grape. Hortic Plant J 4(1):16–23
Chen Z, Zhao J, Qin Y, Hu G (2016) Study on the graft compatibility between ‘Jingganghongnuo’and other litchi cultivars. Sci Hortic 199:56–62
Chen Z, Zhao J, Hu F, Qin Y, Wang X, Hu G (2017) Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile. Sci Rep 7(1):1–12
Flaishman MA, Loginovsky K, Golobowich S, Lev-Yadun S (2008) Arabidopsis thaliana as a model system for graft union development in homografts and heterografts. J Plant Growth Regul 27(3):231–239
Goldschmidt EE (2014) Plant grafting: new mechanisms, evolutionary implications. Front Plant Sci 5:1–9
Haider MS, Zhang C, Kurjogi MM, Pervaiz T, Zheng T, Zhang C, Lide C, Shangguan L, Fang J (2017) Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis. Sci Rep 7(1):1–15
Hao XY, Bi WL, Cui ZH, Pan C, Xu Y, Wang QC (2017) Development, histological observations and Grapevine leafroll-associated virus-3 localisation in in vitro grapevine micrografts. Ann Appl Biol 170(3):379–390
He W, Wang Y, Chen Q, Sun B, Tang HR, Pan DM, Wang XR (2018) Dissection of the mechanism for compatible and incompatible graft combinations of Citrus grandis (L.) Osbeck (‘Hongmian Miyou’). Int J Mol Sci 19(2):1–12
Hudina M, Orazem P, Jakopic J, Stampar F (2014) The phenolic content and its involvement in the graft incompatibility process of various pear rootstocks (Pyrus communis L.). J Plant Physiol 171(5):76–84
Ibacache A, Albornoz F, Zurita-Silva A (2016) Yield responses in Flame Seedless, Thompson Seedless and Red Globe table grape cultivars are differentially modified by rootstocks under semi-arid conditions. Sci Hortic 204:25–32
Irisarri P, Binczycki P, Errea P, Martens HJ, Pina A (2015) Oxidative stress associated with rootstock–scion interactions in pear/quince combinations during early stages of graft development. J Plant Physiol 176:25–35
Iwase A, Mitsuda N, Koyama T, Hiratsu K, Kojima M, Arai T, Ohme-Takagi M (2011) The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. Curr Biol 21(6):508–514
Liu XY, Li J, Liu MM, Yao Q, Chen JZ (2017) Transcriptome profiling to understand the effect of citrus rootstocks on the growth of ‘Shatangju’Mandarin. PLoS One 12(1):1–22
Liu HJ, Zhang JQ, Hu HK, Huang YJ, Xv CM, Hu YY, Huang JQ, Leslie CA, Lou HQ, Zhang QX (2021) Overexpression of JrAMT2 in walnut (Juglans regia L.) rootstock enhances nitrogen level in grafted wild-type walnut scions. Sci Hort 280(109928):1–10
Machado BD, Magro M, Rufato L, Bogo A, Kreztschmar AA (2017) Graft compatibility between european pear cultivars and east malling “C” rootstock. Rev Bras Frutic 39(3):1–9
Melnyk CW, Schuster C, Leyser O, Meyerowitz EM (2015) A developmental framework for graft formation and vascular reconnection in Arabidopsis thaliana. Curr Biol 25(10):1306–1318
Mo Z, Feng G, Su W, Liu Z, Peng F (2018) Identification of miRNAs associated with graft union development in pecan [Carya illinoinensis (Wangenh) K. Koch]. Forests 9(8):1–16
Muawiya AM (2019) In vitro propagation and screening of wild grapevines germplasm for drought tolerance. PMAS- Arid Agriculture University, Rawalpindi (Disseration)
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497
Prabpree A, Sangsil P, Nualsri C, Nakkanong K (2018) Expression profile of phenylalanine ammonia-lyase (PAL) and phenolic content during early stages of graft development in bud grafted Hevea brasiliensis. Biocatal Agric Biotechnol 14:88–95
Reig G, Salazar A, Zarrouk O, Forcada CF, Val J, Moreno MÁ (2019) Long-term graft compatibility study of peach-almond hybrid and plum based rootstocks budded with European and Japanese plums. Sci Hortic 243:392–400
Ren Y, Xu Q, Wang L, Guo S, Shu S, Lu N, Sun J (2018) Involvement of metabolic, physiological and hormonal responses in the graft-compatible process of cucumber/pumpkin combinations was revealed through the integrative analysis of mRNA and miRNA expression. Plant Physiol Biochem 129:368–380
Ribeiro LM, Nery LA, Vieira LM, Mercadante-Simões MO (2015) Histological study of micrografting in passionfruit. Plant Cell Tissue Org Cult 123(1):173–181
Ruffoni B, Airo M, Fascella G, Mascarello C, Zizzo GV, Cervelli C (2003) Propagation and acclimatation of ornamental myrtus genotypes. Acta Hort 616:255–258
Sharma A, Zheng B (2019) Molecular responses during plant grafting and its regulation by Auxins, Cytokinins, and Gibberellins. Biomolecules 9(9):1–20
Shireen F, Nawaz MA, Xiong M, Ahmad A, Sohail H, Chen Z, Abouseif Y, Huang Y, Bie Z (2020) Pumpkin rootstock improves the growth and development of watermelon by enhancing uptake and transport of boron and regulating the gene expression. Plant Physiol Biochem 154:204–218
Songy A, Fernandez O, Clément C, Larignon P, Fontaine F (2019) Grapevine trunk diseases under thermal and water stresses. Planta 249(6):1655–1679
Funding
This research was financially supported by Higher Education Commission of Pakistan funded project no. 6138, Plant Tissue Culture Laboratory, Department of Horticulture, PMAS- Arid Agriculture University Rawalpindi, Pakistan and Public Sector Development Programme (PSDP) funded project No. 321.
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Nazir, F., Ahmad, T., Bashir, M.A. et al. Validation of in vitro grafting using indigenous wild grapevines as rootstock with commercial scion varieties. Acta Physiol Plant 44, 70 (2022). https://doi.org/10.1007/s11738-022-03408-4
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DOI: https://doi.org/10.1007/s11738-022-03408-4