Abstract
Electron beam irradiation (EBI) has protective effects on food storage. This study aimed to investigate drought tolerance of tobacco after EBI treatment. Nicotiana tabacum L. seeds were irradiated with different doses of EBI. Germination percentage (GP) was counted. Phenotype of EBI-treated M1 generation was observed in a drought field. The candidate drought-resistant tobacco line was selected and then drought treated without watering for 8 and 16 days, respectively. Samples at 8 days were collected for RNA-sequencing on the Illumina HiSeqTM 2500 platform. Then, differentially expressed genes (DEGs) were selected, followed by functional enrichment analysis. EBI with 500 Gy was selected as the optimal dose. Five lines: M1-7, M1-9, M1-10, M1-12, and M1-40 were candidate drought-resistant lines based on phenotype observation. Among them, M2-12 and M2-40 had high GPs. By comparing their M1 phenotypes, M2-12 was identified as the final candidate drought-resistant line. After drought treatment, M2-12 showed a wavier leaf margin than control and kept a well growth condition during the whole period. A total of 3547 DEGs were identified, and the up-regulated DEGs were significantly enriched in functions like “response to water deprivation (LOC107782385)”, “response to gibberellin (LOC104231633, LOC104219851)”, and “response to jasmonic acid (JA) (LOC104219851)”. Results of qRT-PCR confirmed that LOC104231633, LOC107782385, and LOC104219851 were up-regulated under drought stress. In conclusion, EBI may be a promising way to generate drought tolerant mutant tobacco. The above three genes may be drought-inducible and may involve in JA- and gibberellin-mediated signaling pathways.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anders S, Huber W (2010) Differential expression analysis for sequence count data. Genome Biol 11:1–12
Ashburner M, Ball CA, Blake JA et al (2000) Gene Ontology: tool for the unification of biology. Nat Genet 25:25–29
Blaszczak AG, Smith R, Gutierrez A, Galbraith DW, Janda J, Vanier C, Wozniak EM (2015) Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. Acta Hortic 1148:85–92
Bombarely A, Menda N, Tecle IY et al (2011) The Sol Genomics Network (solgenomics.net): growing tomatoes using Perl. Nucleic Acids Res 39:1149–1155
Duan Z, Xing Z, Shao Y, Zhao X (2010) Effect of electron beam irradiation on postharvest quality and selected enzyme activities of the white button mushroom, Agaricus bisporus. J Agric Food Chem 58:9617–9621
El-Rehim HAA (2005) Swelling of radiation crosslinked acrylamide-based microgels and their potential applications. Radiat Phys Chem 74:111–117
Frazee AC, Langmead B, Leek JT (2011) ReCount: a multi-experiment resource of analysis-ready RNA-seq gene count datasets. BMC Bioinform 12:449
Frazier TP, Sun G, Burklew CE, Zhang B (2011) Salt and drought stresses induce the aberrant expression of microRNA genes in tobacco. Mol Biotechnol 49:159–165
Harris MA, Clark J, Ireland A et al (2004) The gene ontology (GO) database and informatics resource. Nucleic Acids Res 32:D258–D261
Huang DW, Sherman BT, Tan Q et al (2007) DAVID bioinformatics resources: expanded annotation database and novel algorithms to better extract biology from large gene lists. Nucleic Acids Res 35:169–175
Huang XS, Liu JH, Chen XJ (2010) Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biol 10:230
Kanehisa M, Goto S, Sato Y, Furumichi M, Mao T (2012) KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res 40:D109–D114
Kim J, Chung SO, Jang SA, Jang M, Park CG (2015) Effect of electron beam irradiation on developmental stages of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Radiat Phys Chem 112:139–144
Li F, Xing S, Guo Q et al (2011) Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco. J Plant Physiol 168:960–966
Liu H, Zhou X, Dong N, Liu X, Zhang H, Zhang Z (2011) Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses. Funct Integr Genom 11:431–443
Ma L, Ma Q, Li X, Cheng L, Li K, Li S (2014) Transcriptomic analysis of differentially expressed genes in the Ras1(CA)-overexpressed and wildtype posterior silk glands. BMC Genom 15:106–111
Mahdieh M, Mostajeran A, Horie T, Katsuhara M (2008) Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants. Plant Cell Physiol 49:801–813
Nelson DE, Repetti PP, Adams TR et al (2007) Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA 104:16450–16455
Nir I, Moshelion M, Weiss D (2014) The Arabidopsis gibberellin methyl transferase 1 suppresses gibberellin activity, reduces whole-plant transpiration and promotes drought tolerance in transgenic tomato. Plant Cell Environ 37:113–123
Pereira E, Barreira JCM, Barros L, Antonio AL, Bento A, Ferreira ICFR (2015) Improving the antioxidant activity of medicinal and aromatic plants by applying electron beam irradiation. In: 2nd Symposium on Medicinal Chemistry, Braga
Ptaszek M, Orlikowski LB, Migdał W, Gryczka U (2015) E-beam irradiation for the control of Phytophthora nicotianae var. nicotianae in stonewool cubes. Nukleonika 60:13
Rabara RC, Tripathi P, Lin J, Rushton PJ (2013) Dehydration-induced WRKY genes from tobacco and soybean respond to jasmonic acid treatments in BY-2 cell culture. Biochem Biophys Res Commun 431:409–414
Rabara RC, Tripathi P, Reese RN et al (2015) Tobacco drought stress responses reveal new targets for Solanaceae crop improvement. BMC Genom 16:484
Saad RB, Zouari N, Ramdhan WB, Azaza J, Meynard D, Guiderdoni E, Hassairi A (2010) Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zinc-finger “AlSAP” gene isolated from the halophyte grass Aeluropus littoralis. Plant Mol Biol 72:171–190
Singh N, Mishra A, Jha B (2014) Over-expression of the peroxisomal ascorbate peroxidase (SbpAPX) gene cloned from halophyte Salicornia brachiata confers salt and drought stress tolerance in transgenic tobacco. Mar Biotechnol 16:321–332
Singh SK, Wu Y, Ghosh JS et al (2015) RNA-sequencing reveals global transcriptomic changes in Nicotiana tabacum responding to topping and treatment of axillary-shoot control chemicals. Sci Rep 5:18148
Valero M, Sarrías JA, Alvarez D, Salmerón MC (2006) Modeling the influence of electron beam irradiation on the heat resistance of Bacillus cereus spores. Food Microbiol 23:367–371
Wang JJ, Chen YT, Tian ZJ, Shang FF, Cheng ZJ (2013) Effects of electron beam irradiation on bactericidal efficacy and sensory quality of tobacco leaves. Hubei Agric Sci 12:031
Zhang GF, Wang SJ, Bo-Ji HU, Zhang GF, Shi XM (2003) Primary study on cold tolerant lines of Jojoba by using electron beam irradiation and clone selection. Chin J Oil Crop Sci 25:56–59
Acknowledgements
This work was supported by Scientific and Technological Project of Luoyang Tobacco Company (Project number LYKJ201507).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
All authors declare that they have no conflict of interest to state.
Additional information
Communicated by H Li.
Rights and permissions
About this article
Cite this article
Yang, H., Wang, J., Zhao, L. et al. RNA-sequencing predicts three novel genes in response to drought in electron beam irradiation treated tobacco. Acta Physiol Plant 39, 256 (2017). https://doi.org/10.1007/s11738-017-2553-z
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11738-017-2553-z