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Proteomic changes in response to low-light stress during cotton fiber elongation

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Abstract

Numerous studies have illustrated that low light is one of the major abiotic stresses limiting cotton (Gossypium hirsutum L.) fiber length, but studies addressing molecular mechanisms contributing to reduced fiber growth under low light are lacking. To investigate the molecular mechanisms of cotton fiber elongation in response to low light, an experiment of low light caused by shading was conducted with cotton cultivar NuCOTN 33B. The results showed that low light resulted in shorter fiber length. Proteomic analysis of four developmental stages (5, 10, 15 and 20 days post-anthesis) showed that 49 proteins were significantly responsive to low light. 39 differentially expressed proteins that included some known as well as some novel low-light stress-responsive proteins were identified. These differentially expressed proteins were involved in signal transduction, carbohydrate/energy metabolism, cell wall component synthesis, protein metabolism, cytoskeleton, nitrogen metabolism and stress responses. The results also showed that the decrease in fiber length might be because the levels of signal-related protein (phospholipase D), cytoskeletal proteins (two annexins isoforms), cell wall component-related proteins (sucrose synthase, UDP-d-glucuronic acid 4-epimerase and rhamnose synthase), carbohydrate metabolism-proteins (phosphofructokinase, dihydrolipoamide dehydrogenase, vacuolar H+-ATPase catalytic subunit, malate dehydrogenase and isocitrate dehydrogenase), and stress-related proteins (peroxisomal catalase, short chain alcohol dehydrogenase) were decreased under low light.

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Abbreviations

CRLR:

Crop relative light rate

FB:

Fruiting branch

DPA:

Days post-anthesis

PAR:

Photosynthetic active radiation

2-DE:

Two-dimensional electrophoresis

MDT:

Mean daily temperature

MDTmax:

Mean daily maximum temperature

MDTmin:

Mean daily minimum temperature

LRP:

Low-light-responsive protein

CBL:

Calcineurin B-like protein

PLD:

Phospholipase D

CAP:

Cyclase binding protein

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Acknowledgements

We are grateful for financial support from the National Natural Science Foundation of China (31571606), the Special Fund for Agro-scientific Research in the Public Interest (201303002), Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP) and China Agriculture Research System (CARS-18-20).

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Author notes

  1. Wei Hu and Mi Zheng contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Wei Hu, Mi Zheng, Shanshan Wang, Yali Meng, Youhua Wang, Binglin Chen & Zhiguo Zhou

  2. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Mi Zheng

  3. Department of Crop and Soil Sciences, University of Georgia, Tifton, 31794, USA

    John L. Snider

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  1. Wei Hu
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Correspondence to Zhiguo Zhou.

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Communicated by M Hajduch.

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Hu, W., Zheng, M., Wang, S. et al. Proteomic changes in response to low-light stress during cotton fiber elongation. Acta Physiol Plant 39, 200 (2017). https://doi.org/10.1007/s11738-017-2499-1

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