Abstract
As part of a study to identify potential targets of calcium action in plant cells, a 35-kDa, annexin-like protein was purified from pea (Pisum sativum L.) plumules by a method used to purify animal annexins. This protein, called p35, binds to a phosphatidylserine affinity column in a calcium-dependent manner and binds 45Ca2+ in a dot-blot assay. Preliminary sequence data confirm a relationship for p35 with the annexin family of proteins. Polyclonal antibodies have been raised which recognize p35 in Western and dot blots. Immunofluorescence and immunogold techniques were used to study the distribution and subcellular localization of p35 in pea plumules and roots. The highest levels of immunostain were found in young developing vascular cells producing wall thickenings and in peripheral root-cap cells releasing slime. This localization in cells which are actively involved in secretion is of interest because one function suggested for the animal annexins is involvement in the mediation of exocytosis.
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Abbreviations
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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The authors thank Dennis Brown (Cell Research Institute) for use of this research facilities and Zainab Ilahi and Collin Thomas for valuable technical assistance. Portions of this work were presented at the 1989 and 1990 meetings of the American Society for Cell Biology (Clark et al. 1989, 1990). This work was in part supported by a National Institute of Health Training Grant 1-T32-HD07296-01A3 and by a National Aeronautics and Space Administration grant NAGW 1519.
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Clark, G.B., Dauwalder, M. & Roux, S.J. Purification and immunolocalization of an annexin-like protein in pea seedlings. Planta 187, 1–9 (1992). https://doi.org/10.1007/BF00201617
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DOI: https://doi.org/10.1007/BF00201617