Abstract
Cold shock proteins (CSPs) are a kind of evolutionarily conserved nucleic acid-binding protein widely distributed from prokaryotes to eukaryotes. In this study, BcCSP1, a novel CSP, was isolated from a Pak-choi stress-induced cDNA library by the rapid amplification of cDNA end method. This gene had an open-reading frame (ORF) of 822 base pairs encoding 273 amino acids. BcCSP1 contained an N-terminal CSD domain and a glycine-rich region interspersed with seven CCHC-type zinc fingers at its C terminus. Multi-alignment and phylogenetic analyses showed that BcCSP1 shared high similarity to AtCSP1 and AtCSP3. Real-time polymerase chain reaction analysis showed that BcCSP1 was induced and co-expressed under cold stress and abscisic acid treatments. In addition, a BcCSP1-YFP fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP1 plays an important role in responses to cold and ABA treatments in Pak-choi. This work may be useful for future functional analysis of other CSP genes in Pak-choi.
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References
Chinnusamy V, Zhu J, Zhu J-K (2007) Cold stress regulation of gene expression in plants. Trends Plant Sci 12:444–451
Fusaro AF, Bocca SN, Ramos RLB, Barrôco RM, Magioli C, Jorge VC, Coutinho TC, Rangel-Lima CM, De Rycke R, Inzé D (2007) AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development. Planta 225:1339–1351
Fujita M, Noutoshi Y, Narusaka Y, Shinozaki K (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol 9:436–442
Graham D, Patterson BD (1982) Responses of plants to low, nonfreezing temperatures: proteins, metabolism, and acclimation. Annu Rev Plant Physiol 33:347–372
Graumann P, Marahiel MA (1996) Some like it cold: response of microorganisms to cold shock. Arch Microbiol 166:293–300
Graumann PL, Marahiel MA (1998) A superfamily of proteins that contain the cold-shock domain. Trends Biochem Sci 23:286–290
Juntawong P, Sorenson R, Bailey-Serres J (2013) Cold shock protein 1 chaperones mRNAs during translation in Arabidopsis thaliana. Plant J 74:1016–1028
Karlson D, Imai R (2003) Conservation of the cold shock domain protein family in plants. Plant Physiol 131:12–15
Karlson D, Nakaminami K, Toyomasu T, Imai R (2002) A cold-regulated nucleic acid-binding protein of winter wheat shares a domain with bacterial cold shock proteins. J Biol Chem 277:35248–35256
Kim JS, Park SJ, Kwak KJ, Kim YO, Kim JY, Song J, Jang B, Jung C-H, Kang H (2007) Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coli. Nuleic Acids Res 35:506–516
Kim M-H, Sasaki K, Imai R (2009) Cold shock domain protein 3 regulates freezing tolerance in Arabidopsis thaliana. J Biol Chem 284:23454–23460
Kim M-H, Sato S, Sasaki K, Saburi W, Matsui H, Imai R (2013) COLD SHOCK DOMAIN PROTEIN 3 is involved in salt and drought stress tolerance in Arabidopsis. FEBS Open Bio 3:438–442
Livak KJ, Schmittgen TD (2001) Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods 25:402–408
Nakaminami K, Sasaki K, Kajita S, Takeda H, Karlson D, Ohgi K, Imai R (2005) Heat stable ssDNA/RNA-binding activity of a wheat cold shock domain protein. FEBS Lett 579:4887–4891
Nakaminami K, Karlson DT, Imai R (2006) Functional conservation of cold shock domains in bacteria and higher plants. Proc Natl Acad Sci USA 103:10122–10127
Park SJ, Kwak KJ, Oh TR, Kim YO, Kang H (2009) Cold shock domain proteins affect seed germination and growth of Arabidopsis thaliana under abiotic stress conditions. Plant Cell Physiol 50:869–878
Rajkowitsch L, Chen D, Stampfl S, Semrad K, Waldsich C, Mayer O, Jantsch MF, Konrat R, Blasi U, Schroeder R (2007) RNA chaperones, RNA annealers and RNA helicases. RNA Biol 4:118
Sasaki K, Imai R (2011) Pleiotropic roles of cold shock domain proteins in plants. Front Plant Sci 2:116
Sasaki K, Kim M-H, Imai R (2007) Arabidopsis COLD SHOCK DOMAIN PROTEIN2 is a RNA chaperone that is regulated by cold and developmental signals. Biochem Bioph Res Commun 364:633–638
Sasaki K, Kim MH, Imai R (2013) Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 is a negative regulator of cold acclimation. New Phytol 198:95–102
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Tang J, Wang F, Wang Z, Huang Z, Xiong A, Hou X (2013) Characterization and co-expression analysis of WRKY orthologs involved in responses to multiple abiotic stresses in Pak-choi (Brassica campestris ssp. chinensis). BMC Plant Biol 13:188
Yang Y, Karlson DT (2011) Overexpression of AtCSP4 affects late stages of embryo development in Arabidopsis. J Exp Bot 62:2079–2091
Yang Y, Karlson D (2012) Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion. J Exp Bot 63:4861–4873
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This study was supported by grants from the National Natural Science Foundation of China (31272173).
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Communicated by W. Zhou.
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Huang, F., Tang, J. & Hou, X. Molecular cloning and characterization of BcCSP1, a Pak-choi (Brassica rapa ssp. chinensis) cold shock protein gene highly co-expressed under ABA and cold stimulation. Acta Physiol Plant 38, 47 (2016). https://doi.org/10.1007/s11738-015-2058-6
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DOI: https://doi.org/10.1007/s11738-015-2058-6