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The effect of anthocyanin-rich bilberry extract on the antioxidant system in roots of barley (Hordeum vulgare L.) cultivars under ionizing radiation

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Abstract

The effects of extracted and purified anthocyanin (ACY) from mature berries of Vaccinium myrtillus L., on growth parameters, antioxidant enzymes, total phenolic (TP) content, total flavonoid (TF) content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were investigated in the roots of barley (Hordeum vulgare L.) cultivars Akhisar-98 and Erginel-90 under ionizing irradiation (IR; 10 Gy). The responses to IR and ACY-rich extract treatment in the two cultivars differed; Akhisar-98 was more radiosensitive than Erginel-90 in terms of root development, but IR-induced decreases in Akhisar-98 root length were alleviated by ACY-rich extract (100 µM). Peroxidase (POD) activity in Akhisar and Erginel cultivars decreased, while superoxide dismutase (SOD), glutathione reductase (GR), and ascorbate peroxidase (APX) activities increased. The ACY-rich extract increased POD activity, and decreased SOD, GR, and APX activities in the two barley cultivars. Although TP content increased in the Erginel cultivar more than the Akhisar cultivar, TF contents did not differ between the control and irradiated roots of the Erginel cultivar. Since TP content, TF content, and DPPH radical scavenging activity increased and H2O2 content decreased after ACY-rich extract treatment, ACY-rich extract treatment enhanced antioxidant activity, especially in the Akhisar cultivar. Treatment with ACY-rich extract conferred a greater tolerance to IR stress, especially after irradiation, in the two barley cultivars exposed to IR and exogenic ACY-rich extract. These findings indicate that the Erginel cultivar is more IR-tolerant than the Akhisar cultivar and that exogenous ACY-rich extract treatment promotes antioxidant capacity in barley cultivars under IR stress.

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Acknowledgments

We are grateful to the Aegean Agricultural Research Institute and Gateway Zone Agricultural Research Institute (Turkey) for supplying the Akhisar-98 and Erginel-90 barley cv seeds.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Nesrin Colak & Faik Ahmet Ayaz

  2. Department of Radiation Oncology, Kanuni Training and Research Hospital, 61010, Trabzon, Turkey

    Ahmet Yaşar Zengin

Authors
  1. Nesrin Colak
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  2. Ahmet Yaşar Zengin
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  3. Faik Ahmet Ayaz
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Correspondence to Faik Ahmet Ayaz.

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Communicate by A. Gniazdowska-Piekarska.

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Colak, N., Zengin, A.Y. & Ayaz, F.A. The effect of anthocyanin-rich bilberry extract on the antioxidant system in roots of barley (Hordeum vulgare L.) cultivars under ionizing radiation. Acta Physiol Plant 37, 187 (2015). https://doi.org/10.1007/s11738-015-1925-5

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  • DOI: https://doi.org/10.1007/s11738-015-1925-5

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