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Analysis of biochemical and physiological changes in wheat tissue culture using different germplasms and explant types

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Abstract

To select adequate wheat germplasms for genetic transformation, tissue culture efficiency of 21 different wheat lines (Einkorn, Emmer, Durum wheat, etc.) were compared, along with two different explants, namely, immature embryo and mature embryo. The results showed that the average differentiation rate and regeneration rate of immature embryo calli (46.5 and 20.82 %) were better than those for mature embryo calli (14.03 and 4.37 %). The best genotypes for immature embryo callus culture were ‘Ningchun 16’ and ‘Ei 15’, ‘Xiaoyan 22’, followed by ‘Durum 332’ and ‘Tr 256’. The best genotypes for mature embryo callus culture were ‘Ying 4286’, ‘Yunyin 01’, and ‘Xiaoyan 22’. To analyze how physiological and biochemical settings influence the totipotency of calli, different physiological and biochemical indices were analyzed. Differences between immature embryo callus and mature embryo callus were significant, as well as differences of most indices among different wheat types. The interaction effects between explant types and genotypes were also significant. Correlation analysis results showed that the total phenol and soluble sugar contents were significantly correlated with callus differentiation and regeneration rates.

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References

  • Asahina M, Azuma K, Pitaksaringkarn W, Yamazaki T, Mitsuda N, Ohme-Takagi M, Yamaguchi S, Kamiya Y, Okada K, Nishimura T, Koshiba T, Yokota T, Kamada H, Satoh S (2011) Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in arabidopsis. Proc Natl Acad Sci USA 108(38):16128–16132

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Banno H, Ikeda Y, Niu QW, Chua NH (2001) Overexpression of Arabidopsis ESR1 induces initiation of shoot regeneration. Plant Cell 13:2609–2618

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Barro F, Martin A, Lazzeri PA, Barceló P (1999) Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. Euphytica 108(3):161–167

    Article  Google Scholar 

  • Bi RM, Kou M, Chen LG, Mao SR, Wang HG (2007) Plant regeneration through callus initiation from mature embryo of Triticum. Plant Breeding 126:9–12

    Article  CAS  Google Scholar 

  • Blanc G, Lardet L, Martin A, Jacob J, Carron M (2002) Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.). J Exp Bot 53(373):1453–1462

    Article  CAS  PubMed  Google Scholar 

  • Che P, Lall S, Nettleton D, Howell SH (2006) Gene expression programs during shoot, root, and callus development in arabidopsis tissue culture. Plant Physiol 141(2):620–637

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cui KR, Xing GS, Liu XM, Xing GM, Wang YF (1999) Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbarum L. Plant Sci 146:9–16

    Article  CAS  Google Scholar 

  • Delporte F, Mostade O, Jacquemin JM (2001) Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant Cell Tissue Organ Cult 67(1):73–80

    Article  CAS  Google Scholar 

  • Gao JF (2000) Experiment techniques of plant physiology. World Book Publishing, Xi’an (in Chinese)

    Google Scholar 

  • Hao L, Zhou LN, Xu X, Cao J, Xi T (2006) The role of salicylic acid and carrot embryogenic callus extracts in somatic embryogenesis of naked oat (Avena nuda). Plant Cell Tissue Organ Cult 85:109–113

    Article  CAS  Google Scholar 

  • Harwood WA (2012) Advances and remaining challenges in the transformation of barley and wheat. J Exp Bot 63(5):1791–1798

    Article  CAS  PubMed  Google Scholar 

  • Havaux M, Kloppstech K (2001) The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsis npq and tt mutants. Planta 213(6):953–966

    Article  CAS  Google Scholar 

  • Huang J (2002) Plant biotechnology in China. Science 295(5555):674–676. doi:10.1126/science.1067226

    Article  CAS  PubMed  Google Scholar 

  • Jones HD (2005) Wheat transformation: current technology and applications to grain development and composition. J Cereal Sci 41(2):137–147

    Article  CAS  Google Scholar 

  • Krishnaswamy S, Verma S, Rahman MH, Kav NN (2011) Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis. Plant Mol Biol 75(1–2):107–127

    Article  CAS  PubMed  Google Scholar 

  • Kubeš M, Drážná N, Konrádová H, Lipavská H (2014) Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply. Vitro Cell Dev Biol Plant 50(1):45–57. doi:10.1007/s11627-013-9589-6

    Article  Google Scholar 

  • Lewinsohn E, Gijzen M (2009) Phytochemical diversity: the sounds of silent metabolism. Plant Sci 176(2):161–169

    Article  CAS  Google Scholar 

  • Li HS (2000) Principles and technology of plant physiology and biochemistry experiments. Higher Education Press, Beijing (in Chinese)

    Google Scholar 

  • Lokhande VH, Nikam TD, Penna S (2010) Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L. Plant Cell Tissue Organ Cult 102:17–25

    Article  Google Scholar 

  • Marshall A (2007) GM soybeans and health safety—a controversy reexamined. Nat Biotechnol 25(9):981–987

    Article  CAS  PubMed  Google Scholar 

  • Mathias RJ (1990) Factors affecting the establishment of callus culture in wheat. Biotechnol Agric For 13:24–45

    Google Scholar 

  • Pellegrineschi A, Brito R, Velazquez L, Noguera L, Pfeiffer W, McLean S, Hoisington D (2002) The effect of pretreatment with mild heat and drought stresses on the explant and biolistic transformation frequency of three durum wheat cultivars. Plant Cell Rep 20(10):955–960

    Article  CAS  Google Scholar 

  • Scott KJ, He DJ, Yang YM (1990) Somatic embryo genesis in wheat. Biotechnol Agric For 13:46–67

    Google Scholar 

  • Sun Q, Hu JJ (2006) Plant physiology research and technology. Northwest A&F University Press, Yangling (in chinese)

    Google Scholar 

  • Tao LL, Yin GX, Du LP, Shi ZY, She MY, Xu HJ, Ye XG (2011) Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens. Agric Sci China 10(3):317–326

    Article  CAS  Google Scholar 

  • Wang HB, Fan YL (2006) Researching the mechanism in plant in vitro culture via “communication” experiment and to establish the methods widely used for tissue culture of wheat. Acta Agron Sin 32(7):964–971 (in Chinese)

    CAS  Google Scholar 

  • Wu LM, Wei YM, Zheng YL (2006) Effects of silver nitrate on the tissue culture of immature wheat embryos. Russ J Plant Physiol 53(4):530–534

    Article  CAS  Google Scholar 

  • Yesil-Celiktas O, Nartop P, Gurel A, Bedir E, Vardar-Sukan F (2007) Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis’ calli. J Plant Physiol 164(11):1536–1542. doi:10.1016/j.jplph.2007.05.013

    Article  CAS  PubMed  Google Scholar 

  • Yin GX, Wang YL, She MY, Du LP, Xu HJ, Ma JX, Ye XG (2011) Establishment of a highly efficient regeneration system for the mature embryo culture of wheat. Agric Sci China 10(1):9–17

    Article  CAS  Google Scholar 

  • Yu Y, Wang J, Zhu ML, Wei ZM (2008) Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in China. Plant Breeding 127(3):249–255. doi:10.1111/j.1439-0523.2007.01461.x

    Article  CAS  Google Scholar 

  • Zhang XL, Sun JZ, Liu JL (1992) A comparative study on the biochemical metabolites between the embryogenic and non-embryogenic calli from the variety “Coker 201” of Gossypium hirsutum L. Acta Agron Sin 18(3):176–182 (in Chinese)

    Google Scholar 

  • Zhang XL, Zhang JM, Yiao MM, Sun JZ, Liu JL (1993) Studies on somatic cell culture and its application in cotton (Gossypium L.). J Huazhong Agric Univ 12(5):421–426 (in Chinese)

    Google Scholar 

  • Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J, Xiong AS (2011) Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum L. Mol Biol Rep 38(2):745–753

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by funding from the National Transgene Project, China (2208ZX080022003), and National High Technology Research and Development Program of China (#2011AA100501).

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Authors and Affiliations

  1. Northwest A&F University, 712100, Yangling, Shaanxi, China

    Saiqi Yang, Kaijie Xu, Yongfeng Wang, Bin Bu, Wangqi Huang, Fengli Sun, Shudong Liu & Yajun Xi

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  1. Saiqi Yang
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  2. Kaijie Xu
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  3. Yongfeng Wang
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  4. Bin Bu
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  5. Wangqi Huang
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  7. Shudong Liu
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  8. Yajun Xi
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Correspondence to Yajun Xi.

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Communicated by S. Werbrouck.

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Yang, S., Xu, K., Wang, Y. et al. Analysis of biochemical and physiological changes in wheat tissue culture using different germplasms and explant types. Acta Physiol Plant 37, 120 (2015). https://doi.org/10.1007/s11738-015-1861-4

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