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Comparative effects of NaCl and NaHCO3 stresses on respiratory metabolism, antioxidant system, nutritional status, and organic acid metabolism in tomato roots

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Abstract

Physiological responses of tomato roots to NaCl and NaHCO3 stresses were investigated in a hydroponic setting. The relative growth rate of tomato plants was significantly reduced in both NaCl and NaHCO3 treatments, especially under NaHCO3 stress. Tomato root respiration increased under low concentrations of NaCl and NaHCO3 stresses. However, high concentrations of both NaCl and NaHCO3 significantly inhibited respiration, especially in the NaHCO3 treatment. With increasing concentration of NaCl and NaHCO3 treatment, root Na accumulation increased, while accumulation of N, P, K, Fe, and Mg was significantly lower. Compared to NaCl, NaHCO3 treatment resulted in more dramatic changes in these nutrients. All organic acids investigated were increased by NaHCO3 after 5 days of treatment, but only oxalate, tartrate and malate were induced by NaCl. This implies that global regulation of organic acids might play an important role in tomato’s alkali stress tolerance. Compared to NaCl treatments, NaHCO3 treatments induced much higher levels of reactive oxygen species (ROS) and lipid peroxidation after 5 days of treatment, which was accompanied by higher activities of antioxidant enzymes and higher concentrations of ascorbate–glutathione. However, after 10 days of treatment, 100 mM NaHCO3 stress led to lower accumulation of ROS, antioxidant enzyme activities, and ascorbate–glutathione content. This may have been because root metabolism had almost completely stopped, as indicated by lower root respiration and activity.

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Acknowledgments

This work was supported by the National Basic Research Program of China (No. 2009CB119000) and the China Agriculture Research System (CARS-25-D).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region, Ministry of Agriculture), College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, People’s Republic of China

    Biao Gong, Dan Wen, Xiu Li, Min Wei, Fengjuan Yang, Qinghua Shi & Xiufeng Wang

  2. Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695-7616, USA

    Sean Bloszies

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  1. Biao Gong
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  2. Dan Wen
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  3. Sean Bloszies
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  5. Min Wei
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Correspondence to Qinghua Shi or Xiufeng Wang.

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Communicated by B. Zheng.

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Gong, B., Wen, D., Bloszies, S. et al. Comparative effects of NaCl and NaHCO3 stresses on respiratory metabolism, antioxidant system, nutritional status, and organic acid metabolism in tomato roots. Acta Physiol Plant 36, 2167–2181 (2014). https://doi.org/10.1007/s11738-014-1593-x

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