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Effects of nitrogen source and phosphate concentration on biomass and metabolites accumulation in adventitious root culture of Glycyrrhiza uralensis Fisch

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Abstract

We investigated the influence of ammonia/nitrate ratio and phosphate concentration on biomass and accumulation of metabolites in adventitious roots of Glycyrrhiza uralensis Fisch. A NH4 +/NO3 − ratio of 10:20 was optimal for the production of biomass (0.30 g dry weight) as well as polysaccharide (18.98 mg g−1) and glycyrrhetinic acid (0.31 mg g−1). The content of glycyrrhizic acid (0.47 mg g−1) and flavonoid (8.11 mg g−1) reached the optimum at an ammonia/nitrate ratio of 15:15 and 20:10, respectively. In case of phosphate concentration, a higher growth rate (9.18) and content of polysaccharide (15.66 mg g−1) was obtained at 1.25 mM phosphate concentration. However, 0.625 mM phosphate was favorable for the content of flavonoid (7.54 mg g−1) and glycyrrhizic acid (0.57 mg g−1). The content of glycyrrhetinic acid (0.32 mg g−1) reached the peak when treated with 0.3125 mM phosphate. A scale-up culture of adventitious roots was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of 25.95- and 22.44-fold were obtained in 3 and 5 L BTBBs, respectively, which was higher than that in 0.5 L shake flask (18.36). The contents of flavonoid (7.60 mg g−1) and polysaccharide (15.09 mg g−1) reached the peak in 5 L and 3 L BTBB, respectively. The contents of glycyrrhizic and glycyrrhetinic acid were a little higher than that in BTBBs.

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Acknowledgments

This research was funded by central significant increase or decrease program, China (ID: 20603020302) and Tianjin University innovation fund, China (ID: 2013XQ-0046).

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Authors and Affiliations

  1. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China

    Shuangshuang Yin, Yao Zhang, Wenyuan Gao & Juan Wang

  2. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China

    Shuli Man & Hui Liu

Authors
  1. Shuangshuang Yin
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  2. Yao Zhang
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  3. Wenyuan Gao
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  4. Juan Wang
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  5. Shuli Man
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  6. Hui Liu
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Correspondence to Wenyuan Gao.

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Communicated by K.-Y. Paek.

Yao Zhang is the co-first author.

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Yin, S., Zhang, Y., Gao, W. et al. Effects of nitrogen source and phosphate concentration on biomass and metabolites accumulation in adventitious root culture of Glycyrrhiza uralensis Fisch. Acta Physiol Plant 36, 915–921 (2014). https://doi.org/10.1007/s11738-013-1470-z

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  • DOI: https://doi.org/10.1007/s11738-013-1470-z

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