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Production of flavonoids and polysaccharide by adding elicitor in different cellular cultivation processes of Glycyrrhiza uralensis Fisch

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Abstract

In this study, callus and cell suspension were induced from seedlings of licorice (G. uralensis). In addition, it was revealed that the appropriate concentration of sucrose could promote the callus growth and increase the content of polysaccharide. The methyl jasmonate (MJ) and phenylalanine (PHE) could enhance the callus growth and content of flavonoids for G. uralensis. For producing more flavonoids and polysaccharide, two-stage cultivation was performed. In the first step, 30 g L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day of culture to enhance cell production and metabolite production. In a two-stage cultivation process, PHE (2 mM) and MJ (5 mg L−1) were added into a 5-L balloon-type bubble bioreactor after 10 days of culture. Using a fed-batch cultivation strategy (30 g L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day), polysaccharide production was enhanced to 1.19 g L−1, which was 2.12-fold greater than that in batch cultivation. The flavonoids yield (55.42 mg L−1) which was about 22 % higher than that in batch cultivation was obtained on 21st day. In a two-stage cultivation process, the polysaccharide content was increased by 1.14- and 2.12-fold compared with fed-batch cultivation and batch cultivation on 15th day. Meanwhile, total flavonoids yield (132.36 mg L−1) on 15th day, was increased by 2.26- and 2.67-fold compared with fed-batch cultivation and batch cultivation. In conclusion, two-stage cultivation process combined with the sucrose and elicitor treatment could promote both the callus growth and the secondary metabolites accumulation.

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Abbreviations

MS:

Murashige and Skoog (1962)

BA:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

Naphthalene acetic acid

MJ:

Methyl jasmonate

PHE:

Phenylalanine

w/v:

Weight/volume

vvm:

Air volume/culture volume/min

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Acknowledgments

This research was funded by Science and Technology Commission of Tianjin, China (09ZCKFSH01100).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Songbo Guo, Shuli Man, Wenyuan Gao, Hui Liu & Liming Zhang

  2. Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Songbo Guo, Shuli Man, Wenyuan Gao, Hui Liu & Liming Zhang

  3. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China

    Wenyuan Gao

  4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Peigen Xiao

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  1. Songbo Guo
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  2. Shuli Man
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  3. Wenyuan Gao
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  4. Hui Liu
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  5. Liming Zhang
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Corresponding author

Correspondence to Wenyuan Gao.

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Communicated by K.-Y. Paek.

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Guo, S., Man, S., Gao, W. et al. Production of flavonoids and polysaccharide by adding elicitor in different cellular cultivation processes of Glycyrrhiza uralensis Fisch. Acta Physiol Plant 35, 679–686 (2013). https://doi.org/10.1007/s11738-012-1108-6

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  • DOI: https://doi.org/10.1007/s11738-012-1108-6

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