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Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags

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Abstract

Drought is one of the major abiotic stresses limiting crop productivity in arid and semi-arid regions and influences many aspects of plant development. Groundnut (Arachis hypogaea L.) is an important oil yielding crop and considered as relatively drought tolerant. In this study, two groundnut cultivars were first tested for their drought tolerance based on physiological marker attributes such as relative water content, total chlorophyll content, cell membrane stability and free proline content and identified cultivar K-134 as a drought tolerant and cultivar JL-24 as drought susceptible. To gain a better understanding of the drought stress responses at molecular level, we carried out a genomic analysis of stress-responsive genes/transcripts in drought-tolerant cultivar K-134. As a first step toward characterization of stress-responsive genes, construction and analysis of subtracted cDNA library from drought-tolerant cultivar (K-134) is reported here. Using this strategy a total of 200 ESTs were isolated, sequenced, of which 120 high-quality ESTs were obtained and clustered. Further, our analysis revealed that 31% sequences were unique and no homology to known proteins in the database. This observation has great relevance since groundnut is a stress-adapted legume crop. Further, to validate the identified differentially expressed genes, expression profiles of selected clones were analyzed using dot blot (reverse northern), northern blot analysis. We showed that these clones are differentially expressed under different abiotic stress conditions. The implications of the analyzed genes in abiotic stress tolerance were discussed.

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Acknowledgments

The studies for this publication were conducted as part of the programme ‘Biotechnology for Dry land Agriculture in Andhra Pradesh’’ with financial support of the Research and Communication Division, Ministry of Foreign Affairs, The Government of Netherlands, through APNL-BTU, Hyderabad. We would like to thank Prof. Udayakumar (University of Agricultural Sciences, Bangalore) and Dr. Francoise Cellier (France) for providing clones.

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Authors and Affiliations

  1. Department of Botany, Plant Molecular Biology Unit, Sri Krishnadevaraya University, Anatapur, 515003, India

    G. S. Ranganayakulu, P. Chandraobulreddy, M. Thippeswamy, G. Veeranagamallaiah & Chinta Sudhakar

  2. Department of Botany, Rayalaseema University, Kurnool, 518002, India

    G. S. Ranganayakulu

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  1. G. S. Ranganayakulu
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  2. P. Chandraobulreddy
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  5. Chinta Sudhakar
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Correspondence to Chinta Sudhakar.

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Communicated by Y. Wang.

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Ranganayakulu, G.S., Chandraobulreddy, P., Thippeswamy, M. et al. Identification of drought stress-responsive genes from drought-tolerant groundnut cultivar (Arachis hypogaea L. cv K-134) through analysis of subtracted expressed sequence tags. Acta Physiol Plant 34, 361–377 (2012). https://doi.org/10.1007/s11738-011-0835-4

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  • DOI: https://doi.org/10.1007/s11738-011-0835-4

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