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Long-term maintenance of Pinus nigra embryogenic cultures through cryopreservation

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Abstract

Embryogenic tissues of Pinus nigra have been cryopreserved using a two step slow-freezing method. In the first experiment, 20 cell lines were included and the effect of the duration of cryostorage (1 h vs. 1 year) on regrowth was compared. After a short-term storage (1 h in liquid nitrogen, LN) out of 20 cell lines tested 15 showed regrowth (75%) with individual frequencies 10–100%. Long term storage (1 year in LN) resulted in regrowth of 14 cell lines (70%) while the individual frequencies reached 10–100%. One year storage had no negative influence on the fresh mass accumulation evaluated 2–3 months after thawing. Another 20 cell lines were included in the second experiment with the aim to study the correlation between cryotolerance and maturation capacity of cell lines. Between maturation capacity and cryotolerance expressed as regrowth frequencies of individual cell lines, no correlation has been found.

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Acknowledgments

Financial support from VEGA Grant Agency (project number VEGA 2/0025/08) of the Slovak Academy of Sciences is acknowledged. T.S. also acknowledges also the Research Foundation –Flanders (FWO) for the travel support to Belgium.

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Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39 A, 950 07, Nitra, Slovak Republic

    Terézia Salaj, Ildiko Matušíková & Jan Salaj

  2. Laboratory of Tropical Crop Improvement, K.U. Leuven, Kasteelpark Arenberg 13, 3001, Leuven, Belgium

    Rony Swennen & Bart Panis

  3. Bioversity International, K.U. Leuven, Kasteelpark Arenberg 13, 3001, Leuven, Belgium

    Rony Swennen

Authors
  1. Terézia Salaj
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  2. Ildiko Matušíková
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  3. Rony Swennen
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  4. Bart Panis
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  5. Jan Salaj
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Correspondence to Terézia Salaj.

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Communicated by S. Weidner.

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Salaj, T., Matušíková, I., Swennen, R. et al. Long-term maintenance of Pinus nigra embryogenic cultures through cryopreservation. Acta Physiol Plant 34, 227–233 (2012). https://doi.org/10.1007/s11738-011-0821-x

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  • DOI: https://doi.org/10.1007/s11738-011-0821-x

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