Abstract
A number of morphological, physiological and phenological traits have been suggested as significant markers of adaptation to drought in bread wheat (Triticum aestivum L.). This study was aimed at the identification of a relationship between dehydroascorbate reductase (DHAR, EC 1.8.5.1) and catalase (CAT, EC 1.11.1.6) activities in leaves of wheat plants and stability of yield components under water deficit. The single chromosome substitution lines of cv. Chinese Spring carrying separate chromosomes from the donor Synthetic 6x, an artificial hexaploid combining the genomes of the two wild species, Triticum dicoccoides (AABB) and Aegilops tauschii (DD), were the objects of the investigations. The activities of the DHAR and CAT were correlated with flag leaf relative water content and two indexes of stability of grain yield components under drought across the set substitution lines. The lines carrying a synthetic hexaploid homologous pair of chromosomes 1B, 1D, 2D, 3D or 4D all expressed a low constitutive level of DHAR and the lines carrying chromosomes 3B, 1D, 2D and 3D a low constitutive level of CAT. All were able to increase this level (by fourfold for DHAR and by 1.5-fold for CAT) in response to stress caused by water deficit. When challenged by drought stress, these lines tended to be the most effective in retaining the water status of the leaves and preventing the grain yield components from being compromised. The discovered genetic variability for enzymes activity in leaves of wheat might be a useful selection criterion for drought tolerance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
Baier M, Noctor G, Foyer C, Dietz K-J (2000) Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. Plant Physiol 124:823–832
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413–428
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought—from genes to the whole plant. Funct Plant Biol 30:239–264
Chen Z, Gallie DR (2004) The ascorbic acid redox state controls guard cell signaling and stomatal movement. Plant Cell 16:1143–1162
Chen Z, Gallie DR (2006) Dehydroascorbate reductase affects leaf growth, development, and function. Plant Physiol 142:775–787
Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision breeding in the twenty-first century. Philos Trans R Soc B 363:557–572
Collins NC, Tardieu F, Tuberosa R (2008) Quantitative trait loci and crop performance under abiotic stress: where do we stand? Plant Physiol 147:469–486
Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427
Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars. I. Grain yield responses. Aust J Agric Res 29:897–917
Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over last 20 years. Euphytica 156:1–13
Halloran GM, Ogbonnaya FC, Lagudan ES (2008) Triticum (Aegilops) tauschii in natural and artificial synthesis of hexaploid wheat. Aust J Agric Res 59:475–490
Jackson EA, Holt LM, Payne PI (1983) Characterisation of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and chromosomal localization of their controlling genes. Theor Appl Genet 66:29–37
Khlestkina EK, Röder MS, Salina EA (2008) Relationship between homoeologous regulatory and structural genes in allopolyploid genome—a case study in bread wheat. BMC Plant Biol 8:88
Kohler B, Hills A, Blatt MR (2003) Control of guard cell ion channels by hydrogen peroxide and abscisic acid indicates their action through alternate signaling pathways. Plant Physiol 131:385–388
Kuol BG (2004) Breeding for drought tolerance in sesame (Sesamum indicum L.) in Sudan.Cuvillier, Göttingen, p 224
Luna CM, Pastory GM, Driscoll S, Groten K (2005) Drought control on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. J Exp Bot 56:417–423
McFadden ES, Sears ER (1946) The origin of Triticum spelta and its free-threshing hexaploid relative. J Hered 37:81–89
Pastori GM, Foyer CH (2002) Common components, networks and pathways of cross-tolerance to stress. The central role of “redox” and abscisic acid-mediated controls. Plant Physiol 129:460–468
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor App Genet 58:113–120
Pei ZM, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signaling in guard cells. Nature 406:731–734
Pestsova EG, Börner A, Röder MS (2001) Development of a set of Triticum aestivum/Aegilops tauschii introgression lines. Hereditas 135:139–143
Pestsova EG, Börner A, Röder MS (2006) Development and QTL assessment of Triticum aestivum-Aegilops tauschii introgression lines. Theor App Genet 112:634–647
Praba ML, Cairns JE, Babu RC, Lafitte HR (2009) Identification of physiological traits underlying cultivar differences in drought tolerant wheat. J Agric Crop Sci 195:30–46
Rampino P, Pataleo S, Gerardi C, Mita G, Perrotta C (2006) Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell Environ 29:2143–2152
Richards RA, Rebetzke GJ, Condon AG, Watt M, Spielmeyer W, Ellis MH, Bonnett DG, Dolferus R (2008) Genetic improvement of wheat for dry environments—trait based approach. In: Appels R, Eastwood R et al (eds) Proceedings of 11th IWGS. Sydney University Press, Sydney
Richards RA, Rebetzke GJ, Watt M, Condon AGT, Spielmeyer W, Dolferus R (2010) Breeding for improved water productivity in temperate cereals: phenotyping, quantitative trait loci, markers and the selection environment. Funct Plant Biol 37:85–97
Ried JL, Walter-Simmons MK (1993) Group 3 late embryogenesis abundant proteins in desiccation-tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiol 102:125–131
Salina E, Korzun V, Pestsova E, Röder M, Börner A (2003) The study of authenticity of three sets of inter-varietal substitution lines of wheat (Triticum aestivum L.). In: EWAC Newsletter, Proceedings 12th International EWAC Workshop, 1–6 July 2002, John Innes Centre, Noriwich, pp 28–31
Varshney RK, Tuberosa R (eds) (2008) Genomic-Assisted Crop Improvement, V.2. Genomics applications in crops. Springer, Berlin, p 509
Woodbury W, Spencer AK, Stahmann MA (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem 44:301–305
Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, Song CP (2001) Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448
Zhurbitzkiy ZI (1968) Theory and practice of vegetation method. Nauka, Moscow, p 260
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by R. Aroca.
Rights and permissions
About this article
Cite this article
Osipova, S.V., Permyakov, A.V., Permyakova, M.D. et al. Leaf dehydroascorbate reductase and catalase activity is associated with soil drought tolerance in bread wheat. Acta Physiol Plant 33, 2169–2177 (2011). https://doi.org/10.1007/s11738-011-0756-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11738-011-0756-2