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Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza

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Abstract

A new putative gene encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase (designated as SmHMGS, GenBank Accession No. FJ785326), which catalyses the condensation of acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA as an early step in the mevalonic acid pathway, was isolated from young leaves of Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of the putative SmHMGS was 1,655 bp containing a 1,381 bp open reading frame (ORF) encoding a polypeptide of 460 amino acids. Comparative and bioinformatic analyses revealed that SmHMGS showed extensive homology with HMGSs from other plant species. Phylogenetic tree analysis indicated that SmHMGS belonged to the plant HMGS super family and had the closest relationship with HMGS from Hevea brasiliensis. Tissue expression pattern analysis revealed that the putative SmHMGS was constitutively expressed in all the tested tissues and strong in leaf, moderate in stem, weak in root, which was in contrast to SmHMGR reported before. The putative SmHMGS was found to be an elicitor-responsive gene, which could be induced by exogenous elicitors, including salicylic acid (SA) and methyl jasmonate (MJ). These results will help in understanding the role of HMGS in tanshinones biosynthesis in S. miltiorrhiza.

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References

  • Alex D, Bach TJ, Chye ML (2000) Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive. Plant J 22:415–426

    Article  PubMed  CAS  Google Scholar 

  • Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48:355–381

    Article  PubMed  CAS  Google Scholar 

  • Eisenreich W, Rohdich F, Bacher A (2001) Deoxyxylulose phosphate pathway to terpenoids. Trends Plant Sci 6(2):78–84

    Article  PubMed  CAS  Google Scholar 

  • Ge XC, Wu JY (2005a) Induction and potentiation of diterpenoid tanshinone accumulation in Salvia miltiorrhiza hairy roots by β-aminobutyric acid. Microbiol Biotechnol 68:183–188

    Article  CAS  Google Scholar 

  • Ge XC, Wu JY (2005b) Tanshinone production and isoprenoid path ways in Salvia miltiorrhiza hairy roots induced by Ag+ and yeast elicitor. Plant Sci 168:487

    Article  CAS  Google Scholar 

  • Hayat S, Ahmad A (2007) Salicylic acid: a plant hormone. Springer, The Netherlands, ISBN 1402051832

  • Hu P, Luo GA, Zhao ZZ, Jiang ZH (2005) Quality assessment of Radix salvia miltiorrhiza. Chem Pharm Bull 53:481–484

    Article  PubMed  CAS  Google Scholar 

  • Huang B, Yi B, Duan Y, Sun L, Yu X, Guo J, Chen W (2008) Characterization and expression profiling of tyrosine aminotransferase gene from Salvia miltiorrhiza (Dan-shen) in rosmarinic acid biosynthesis pathway. Mol Biol Rep 35:601–612

    Article  PubMed  CAS  Google Scholar 

  • Jiang JH, Kai GY, Cao XY, Chen FM, He DN, Liu Q (2006) Molecular cloning and function identification of a new HMG-CoA reductase gene from Eucommia ulmoides Olive. Biosci Rep 26(2):171–181

    Article  PubMed  CAS  Google Scholar 

  • Kai GY, Miao ZQ, Zhang L, Zhao DL, Liao ZH, Sun XF, Zhao LX, Tang KX (2006) Molecular cloning and expression analyses of a new gene encoding 3-hydroxy-3-methylglutaryl -CoA synthase from Taxus × media. Biol Plant 50(3):359–366

    Google Scholar 

  • Kai GY, Liao P, Zhang T, Zhou W, Wang J, Xu H, Liu Y, Zhang L (2010) Characterization, expression profiling and functional identification of a gene encoding geranylgeranyl diphosphate synthase from Salvia miltiorrhiza. Biotechnol Bioprocess Eng 15 (in press)

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 12:1244–1245

    Article  Google Scholar 

  • Lange BM, Rujan T, Martin W, Croteau R (2000) Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes. Proc Natl Acad Sci USA 97:13172–13177

    Article  PubMed  CAS  Google Scholar 

  • Li L, Hu GL, Frank SCL, Wang XR (2001) Content and distribution characteristics of anti-oxidant components from Salvia miliorrhiza Bge. Acta Agric Univ Jiangxiensis 23:487–491 (in Chinese)

    CAS  Google Scholar 

  • Li QF, Shi SL, Liu QR, Tang J, Song J, Liang Y (2008) Anticancer effects of ginsenoside Rg1, cinnamic acid, and tanshinone IIA in osteosarcoma MG-63 cells: nuclear matrix downregulation and cytoplasmic trafficking of nucleophosmin. Int J Biochem Cell Biol 40(9):1918–1929

    Article  PubMed  CAS  Google Scholar 

  • Liao P, Zhou W, Zhang L, Wang J, Yan XM, Zhang Y, Zhang R, Li L, Zhou GY, Kai GY (2009) Molecular cloning, characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Salvia miltiorrhiza. Acta Physiol Plant 31(3):565–572

    Article  CAS  Google Scholar 

  • Lu Y, Wang HS, Wang W, Qian ZY, Li L, Wang J, Zhou GY, Kai GY (2009) Molecular characterization and expression analysis of a new cDNA encoding strictosidine synthase from Ophiorrhiza japonica. Mol Biol Rep 36:1845–1852

    Article  PubMed  CAS  Google Scholar 

  • Montamat F, Guilloton M, Karst F, Delrot S (1995) Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase. Gene 167:197–201

    Article  PubMed  CAS  Google Scholar 

  • Nagegowda D, Bach TJ, Chye ML (2004) Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes. Biochem J 383:517–527

    Article  PubMed  CAS  Google Scholar 

  • Nizamutdinova IT, Lee GW, Lee JS, Cho MK, Son KH, Jeon SJ, Kang SS, Kim YS, Lee JH, Seo HG, Chang KC, Kim HJ (2008) Tanshinone I suppresses growth and invasion of human breast cancer cells, MDA-MB-231 through regulation of adhesion molecules. Carcinogenesis 29(10):1885–1892

    Article  PubMed  CAS  Google Scholar 

  • Pauwels L, Morreel K, Witte ED, Lammertyn F, Montagu MV, Boerjan W, Inzé D, Goossens A (2008) Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells. Proc Natl Acad Sci USA 105:1380–1385

    Google Scholar 

  • Rao MV, Davis KR (1999) Ozone-induced cell death occurs via two distinct mechanisms in Arabidopsis: the role of salicylic acid. Plant J 17:603–614

    Article  PubMed  CAS  Google Scholar 

  • Senaratna T, Touchell D, Bunn E, Dixon K (2000) Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul 30:157–161

    Article  CAS  Google Scholar 

  • Sirinupong N, Suwanmanee P (2005) Molecular cloning of a new cDNA and expression of 3-hydroxy-3-methylglutaryl-CoA synthase gene from Hevea brasiliensis. Planta 221(4):502–512

    Article  PubMed  CAS  Google Scholar 

  • Suwanmanee P, Suvachittanont W, Fincher GB (2002) Molecular cloning and sequencing of a cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase from Hevea brasillensis (HBK). Mull Sci Asia 28:29–36

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Wang XY, Cui GH, Huang LQ, Qiu DY (2007) Effects of methyl jasmonate on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root. Zhongguo Zhong Yao Za Zhi 32:300–302

    PubMed  CAS  Google Scholar 

  • Wasternack C, Parthier B (1997) Jasmonate-signalled plant gene expression. Trends Plant Sci 2(8):302–307

    Article  Google Scholar 

  • Wegener A, Gimbel W, Werner T, Hani J, Ernst D, Sandermann HJ (1997) Molecular cloning of ozone-inducible protein from Pinus sylvestris L. with high sequence similarity to vertebrate 3-hydroxy-3-methylglutaryl- CoA-synthase. Biochim Biophys Acta 1350:247–252

    PubMed  CAS  Google Scholar 

  • Yan Q, Hu Z, Tan RX, Wu JY (2005) Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation. J Biotechnol 119:416–424

    Article  PubMed  CAS  Google Scholar 

  • Yan XM, Zhang L, Wang J, Liao P, Zhang Y, Zhang R, Kai GY (2009) Molecular characterization and expression of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) gene from Salvia miltiorrhiza. Acta Physiol Plant 31(5):1015–1022

    Google Scholar 

  • Zhang C, Liu L, Xu H, Wei ZY, Wang YL, Lin YJ, Gong WM (2007) Crystal structures of human IPP isomerase new insights into the catalytic mechanism. J Mol Biol 366(5):1437–1446

    Article  PubMed  CAS  Google Scholar 

  • Zhang FY, Zheng WH, Pi RB, Mei ZR, Bao YX, Gao J, Tang WJ, Chen SR, Liu PQ (2009) Cryptotanshinone protects primary rat cortical neurons from glutamate-induced neurotoxicity via the activation of the phosphatidylinositol 3-kinase/Akt signaling pathway. Exp Brain Res 193(1):109–118

    Article  PubMed  CAS  Google Scholar 

  • Zhou L, Zuo Z, Chow MS (2005) Danshen: an overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J Clin Pharmacol 45:1345–1359

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by Zhejiang Provincial Natural Science Fund (Y2080621), National Natural Science Fund (30900110), Shanghai Education Committee Fund (09ZZ138, J50401, 06DZ015), Project from Ministry of Science and Technology of China (NC2010AE0075; NC2010AE0372), Shanghai Science and Technology Committee Project (10JC1412000, 09QH1401900, 06QA14038, 08391911800, 073158202, 075405117, 065458022, 05ZR14093), National Transgenic Organism New Variety Culture Key Project (2009ZX08012-002B), Fujian Science and Technology Committee Key Special Project (2008NZ0001-4), Project from Shanghai Normal University (SK200830, CH030).

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Authors and Affiliations

  1. Department of Pharmacy, Shaoxing People’s Hospital, 312000, Shaoxing, People’s Republic of China

    Lin Zhang

  2. Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234, Shanghai, People’s Republic of China

    Xiangming Yan, Jing Wang, Shanshan Li, Pan Liao & Guoyin Kai

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  1. Lin Zhang
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  2. Xiangming Yan
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  3. Jing Wang
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  4. Shanshan Li
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  5. Pan Liao
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  6. Guoyin Kai
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Correspondence to Guoyin Kai.

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Communicated by L. A. Kleczkowski.

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Zhang, L., Yan, X., Wang, J. et al. Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza . Acta Physiol Plant 33, 953–961 (2011). https://doi.org/10.1007/s11738-010-0627-2

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  • DOI: https://doi.org/10.1007/s11738-010-0627-2

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