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Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum

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Abstract

Interspecific somatic hybrids between a diploid potato clone DG 81-68 susceptible to Phytophthora infestans (Mont.) de Bary and a resistant diploid tuber-bearing species Solanum × michoacanum were generated and analyzed. About 30 regenerants displaying an intermediate morphology were obtained as a result of three separate PEG-mediated fusion experiments. The RAPD analysis confirmed the hybridity of all the regenerants. About 50% of the hybrid plants exhibited vigorous growth and were stable in culture, while the rest of them rooted poorly and grew slowly in vitro. Most of the hybrid clones were at the tetraploid level (70%), while 30% of the clones examined were at the hexaploid level. The S. × michoacanum (+) DG 81-68 hybrids with growth anomalies were aneuploid. The variation in late blight resistance of the hybrid clones was found in detached leaflet tests, with enhanced resistance characteristic for three tetraploid hybrids.

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Acknowledgments

The authors are grateful to Ms. I. Dzikowska for propagation of in vitro plants.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106, Warsaw, Poland

    Anna Szczerbakowa, Justyna Tarwacka & Bernard Wielgat

  2. The Emil Chroboczek Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100, Skierniewice, Poland

    Michał Oskiera

  3. Młochów Research Center, Plant Breeding and Acclimatization Institute (IHAR), Platanowa 19, 05-831, Młochów, Poland

    Henryka Jakuczun

Authors
  1. Anna Szczerbakowa
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  2. Justyna Tarwacka
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  3. Michał Oskiera
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  4. Henryka Jakuczun
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  5. Bernard Wielgat
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Correspondence to Anna Szczerbakowa.

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Communicated by J. Sadowski.

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Szczerbakowa, A., Tarwacka, J., Oskiera, M. et al. Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum . Acta Physiol Plant 32, 867–873 (2010). https://doi.org/10.1007/s11738-010-0472-3

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  • DOI: https://doi.org/10.1007/s11738-010-0472-3

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