Abstract
The suadea salsa full-length S-adenosylmethionine synthetase (SsSAMS2) was introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens-mediated transformation. The gene transformation and expression in tobacco were confirmed by PCR, RT-PCR and Northern blotting analysis. Several transgenic lines (ST lines) overexpressing SsSAMS2 gene under the control of cauliflower mosaic virus 35S promoter showed more seeds number and weight, and accumulated higher free total polyamines (PAs) than wild-type plants (WT lines) and transformants with blank vector (BT lines). Salt stress-induced damage was attenuated in these transgenic plants, in the symptom of maintaining higher photosynthetic rate and biomass. These results that the transgenic plants overexpressing suadea salsa SAMS2 are more tolerant to salt stress than wild-type plants suggest that PAs may play an important role in contributing salt tolerance to plants.
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Abbreviations
- PAs:
-
Polyamines
- SAMS:
-
S-adenosylmethionine synthetase
- WT:
-
Wild-type tobacco
- ST:
-
Transgenic lines with with suadea salsa SAMS2 cDNA
- BT:
-
Transgenic lines with blank vector
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This work was supported by the Hi-Tech Research and Development (863) Program of China (2002AA629080) and the State Key Basic Research and Development Plan of China (G1995011700).
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Communicated by L.A. Kleczkowski.
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Qi, YC., Wang, FF., Zhang, H. et al. Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco. Acta Physiol Plant 32, 263–269 (2010). https://doi.org/10.1007/s11738-009-0403-3
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DOI: https://doi.org/10.1007/s11738-009-0403-3