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Acta Physiologiae Plantarum

, Volume 31, Issue 3, pp 487–494 | Cite as

Micropropagation of Swertia chirata Buch.-Hams. ex Wall.: a critically endangered medicinal herb

  • K. Balaraju
  • P. AgastianEmail author
  • S. Ignacimuthu
Original Paper

Abstract

An efficient in vitro plant regeneration protocol for Swertia chirata Buch.-Ham. ex Wall (Gentianaceae), a critically endangered Himalayan medicinal herb, was developed using shoot tip explants derived from in vitro grown seedlings. Media with 2% sucrose and various types of hormones markedly influenced in vitro propagation of S. chirata. An in vitro shootlet production system using Murashige and Skoog (MS) medium with various hormones such as BAP, KN and TDZ was established. BAP at 1.0 mg/l and KN, 0.1 mg/l induced highest number of multiple shoots (42.16 ± 1.05) per explant. Micro-proliferated shoots were transferred to elongation medium amended with GA3 (0.1 mg/l) and hormone free basal medium, after which they were transferred to rooting medium. The highest frequency of rooting (22.48 ± 1.08) was obtained in half-strength MS medium supplemented with NAA, 0.1 mg/l after testing with different auxins at various concentrations within 4 weeks of transfer to the rooting medium. Hardening was successfully attained under controlled conditions inside the plant tissue culture room. This method could effectively be applied for the conservation and clonal propagation to meet the pharmaceutical demands.

Keywords

Swertia chirata Shoot tip MS medium Multiple shooting 

Abbreviations

BAP

6-Benzylaminopurine

2,4-D

2,4-Dichlorophenoxyacetic acid

IBA

Indole-3-butyric acid

IAA

Indole-3-acetic acid

KN

Kinetin

NAA

α-Naphthalene acetic acid

TDZ

Thidiazuron

Notes

Acknowledgment

The authors are grateful to the University Grants Commission, New Delhi, India, for providing financial assistance (Sanction No. F.31-238/2005(SR) dated 05.04.2006).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  1. 1.Research Department of Plant Biology and Biotechnology, School of Life SciencesLoyola CollegeChennaiIndia
  2. 2.Entomology Research InstituteLoyola CollegeChennaiIndia

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