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Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

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Abstract

This paper discusses a number of experiments performed, involving the fusion by an electric field of mesophyll protoplasts from Solanum tuberosum cv. Bintje, S. tuberosum dihaploid clones 243, 299 and the wild tuberous disease-resistant species S. bulbocastanum and S. pinnatisectum. Three fusion experiments (S. bulbocastanum + S. tuberosum dihaploid 243, S. pinnatisectum + S. tuberosum cv. Bintje and S. pinnatisectum + S. tuberosum dihaploid 299) yielded 542 calli, the 52 ones of which produced shoots. Obtained regenerants were estimated by the flow-cytometry (FC) and RAPD analysis to determine hybrid plants.The utilisation of the FC as a useful method for detecting somatic hybrids is also discussed in this paper. The combination S. bulbocastanum + S. tuberosum dihaploid 243 led to the creation of eight somatic hybrids, the combination S. pinnatisectum + S. tuberosum cv. Bintje yielded four somatic hybrids and the combination S. pinnatisectum + S. tuberosum dihaploid 299 resulted in no hybrid regenerants. Morphology in vitro, growth vigour and production of tuber-like structures were evaluated in hybrid plants. Plants were transferred in vivo for further estimation (acclimatization, habitus evaluation and tuberization ability).

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Acknowledgments

The authors thank to Institute of Experimental Botany Academy of Sciences of the Czech Republic namely to Pavla Suchánková and David Kopecký for excellent FC analyses. The autors are grateful to Taisia Zatsepin for language emendation. This work was supported by project NAZV QF 4133 and MSMT MSM6010980701 of the Czech Republic.

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  1. Laboratory of Experimental Breeding, Potato Research Institute Havlíčkův Brod, Ltd., Dobrovského 2366, 580 01, Havlíckuv Brod, Czech Republic

    Marie Greplová, Hana Polzerová & Hana Vlastníková

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  1. Marie Greplová
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  2. Hana Polzerová
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  3. Hana Vlastníková
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Correspondence to Marie Greplová.

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Communicated by M. Stobiecki.

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Greplová, M., Polzerová, H. & Vlastníková, H. Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum . Acta Physiol Plant 30, 787–796 (2008). https://doi.org/10.1007/s11738-008-0183-1

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