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Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase

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Abstract

The influence of Putrescine (Put) on the growth and elicitation of anthocyanin in callus cultures of Daucus carota var. Nantes scarlet was investigated through the use of α-DL-difluoromethylarginine (DFMA), the polyamine (PA) biosynthetic inhibitor. It was observed that the addition of Put (0.05 mM) resulted in enhancement of growth and anthocyanin content. The anthocyanin content was found to be enhanced by 1.68 fold on the 21st day as compared to the untreated controls. The PA inhibitor was found to result in lowering of the growth and the anthocyanin accumulation, which could be partially restored by the addition of Put in combination with this inhibitor. The levels of Ca2+ ATPase were also found to be elevated in treatment with Put suggesting the involvement of calcium in the elicitation of anthocyanin. The endogenous titres of PAs and the ethylene production under these treatments were also studied. The treatment with DFMA resulted in lower levels of endogenous PAs and higher levels of ethylene. Lowering of ethylene by putrescine treatment shows that PA treatment also inhibited ethylene formation, which would also imply that endogenous ethylene does not influence anthocyanin production in carrot callus cultures.

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Abbreviations

PAs:

Polyamines

Put:

Put-rescine

Spd:

Spermidine

Spm:

Spermine

DFMA:

α-DL-difluoromethylarginine

DFMO:

α-DL-difluoromethylornithine

SAM:

S-adenosylmethionine

ODC:

Ornithine decarboxylase

ACC:

aminocyclopropane-carboxylic acid

2,4-D:

2,4-Dichlorophenoxy acetic acid

Kn:

Kinetin

IAA:

Indole 3- acetic acid

PCA:

perchloric acid

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Authors and Affiliations

  1. Department of Plant Cell Biotechnology, Central Food Technological Research Institute, 570 013, Mysore, India

    Sudha G. & Ravishankar G. A.

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  1. Sudha G.
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  2. Ravishankar G. A.
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Correspondence to Ravishankar G. A..

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Sudha, G., Ravishankar, G.A. Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase. Acta Physiol Plant 25, 69–75 (2003). https://doi.org/10.1007/s11738-003-0038-8

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  • DOI: https://doi.org/10.1007/s11738-003-0038-8

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