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Efficient acetoin production from pyruvate by engineered Halomonas bluephagenesis whole-cell biocatalysis

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Abstract

Acetoin is an important platform chemical, which has a wide range of applications in many industries. Halomonas bluephagenesis, a chassis for next generation of industrial biotechnology, has advantages of fast growth and high tolerance to organic acid salts and alkaline environment. Here, α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis 168 were co-expressed in H. bluephagenesis to produce acetoin from pyruvate. After reaction condition optimization and further increase of α-acetolactate decarboxylase expression, acetoin production and yield were significantly enhanced to 223.4 mmol·L−1 and 0.491 mol·mol−1 from 125.4 mmol·L−1 and 0.333 mol·mol−1, respectively. Finally, the highest titer of 974.3 mmol·L−1 (85.84 g·L−1) of acetoin was accumulated from 2143.4 mmol·L−1 (188.6 g·L−1) of pyruvic acid within 8 h in fed-batch bioconversion under optimal reaction conditions. Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that the whole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L−1 (38.2, 38.8, 33.4, 39.0 and 29.9 g·L−1) acetoin in five repeated cycles under the same conditions. This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad development prospect in biosynthesis of acetoin.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFA0900 200) and the National Natural Science Foundation of China (Grant No. NSFC-21621004). We thank Prof. Guo-Qiang Chen from Tsinghua University for generously providing experimental materials.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    Meiyu Zheng, Zhenzhen Cui, Jing Zhang, Zhiwen Wang & Tao Chen

  2. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    Meiyu Zheng, Zhenzhen Cui, Jing Zhang, Zhiwen Wang & Tao Chen

  3. Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden

    Jing Fu

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  1. Meiyu Zheng
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Correspondence to Tao Chen.

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Zheng, M., Cui, Z., Zhang, J. et al. Efficient acetoin production from pyruvate by engineered Halomonas bluephagenesis whole-cell biocatalysis. Front. Chem. Sci. Eng. 17, 425–436 (2023). https://doi.org/10.1007/s11705-022-2229-0

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